Adenine-coated magnetic multiwalled carbon nanotubes for the selective extraction of aristolochic acids based on multiple interactions
Adenine-coated magnetic multiwalled carbon nanotubes for the selective extraction of aristolochic acids based on multiple interactions
复制标题
基于多重相互作用的腺嘌呤涂层磁性多壁碳纳米管选择性提取马兜铃酸
DOI：
10.1016/j.chroma.2020.461382
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发表时间：
2020
影响因子：
4.1
通讯作者：
Fu Qiang
中科院分区：
文献类型：
--
作者：
Shu Hua;Chen Guoning;Wang Lu;Cui Xia;Wang Qun;Li Wen;Chang Chun;Guo Qi;Luo Zhimin;Fu Qiang
研究了磁性碳纳米管功能化对马兜铃酸Ⅰ和Ⅱ的识别方法。3-以缩水甘油基氧丙基三甲氧基硅烷为偶联剂，将腺嘌呤偶联到固体载体上。利用透射电子显微镜（TEM）、扫描电子显微镜（SEM）、X射线衍射仪（XRD）和振动样品磁强计（VSM）对磁性碳纳米管的形貌和结构进行了研究。通过吸附等温线、吸附动力学和吸附选择性测试，评价了磁性碳纳米管对腺嘌呤的吸附性能。腺嘌呤功能化吸附剂对马兜铃酸Ⅰ的吸附容量为24.5 μg mg−1。建立了磁性固相萃取-高效液相色谱检测方法，用于中药中马兜铃酸的富集和检测。马兜铃酸Ⅰ和马兜铃酸Ⅱ的回收率分别为92.7 - 97.5%和92.6 - 99.4%，相对标准偏差小于4.0%。
A method is described for the functionalization of magnetic carbon nanotubes to recognize aristolochic acid Ⅰ and Ⅱ. 3-Glycidyloxypropyltrimethoxysilane was used as a coupling agent to immobilize adenine on a solid support. The morphology and structure of adenine-coated magnetic carbon nanotubes was investigated using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and a vibrating sample magnetometer (VSM). The adsorption performance of the adenine-coated magnetic carbon nanotubes was evaluated via adsorption isotherms, the kinetics and selectivity tests. The adsorption capacity of the adenine-functionalized sorbent for aristolochic acid Ⅰ was determined to be 24.5 μg mg−1. By combining magnetic solid phase extraction with HPLC detection, a method was developed to enrich and detect aristolochic acids used in traditional Chinese medicine. A satisfactory recovery (92.7 - 97.5% for aristolochic acid Ⅰ and 92.6 - 99.4% for aristolochic acid Ⅱ) and an acceptable relative standard deviation (<4.0%) were obtained.