Biocompatibility of Implant Materials: Cell Culture Assay
Biocompatibility of Implant Materials: Cell Culture Assay
复制标题
植入材料的生物相容性：细胞培养测定
DOI：
10.1007/978-94-011-7432-9_11
复制
发表时间：
1985
影响因子：
2.4
通讯作者：
M. Amedeo
中科院分区：
文献类型：
--
作者：
M. Cannas;F. Denicolai;G. Tarone;M. Amedeo
研究置换手术中使用的材料（生物材料）的身体部位的“可接受性”需要“体外”初步实验，在此期间，观察人（1）或动物（2）细胞系的生长和与生物材料的粘附过程，以确定其可接受性。随后，进行“体内”测试以评估诸如植入物的形状、其所经受的应力以及身体的一般和局部反应机制等变量。本文报道了成纤维细胞在钝化钛、钛合金和四种玻璃（B4 ~ B7）上生长和粘附的定量研究。用从人血浆中纯化的纤连蛋白包被相同的材料后，也进行了研究。扫描电子显微镜（SEM）用于研究细胞生长的形态模式。
Study of the “acceptability” by parts of the body of materials used in replacement operations (biomaterials) requires “in vitro” preliminary experimentation, during which human (1) or animal (2) cell lines are observed in the course of their growth and adhesion to biomaterials to determine their acceptability. Subsequently, “in vivo” tests are run to assess such variables as the shape of the implant, the stresses to which it is subjected and the body’s general and local response mechanisms. This paper reports a quantitative study of the growth and adhesion of fibroblasts on specimens of passivated titanium, titanium alloy and four types of bioglass (B4 to B7). A-study was also made of the same materials following their coating with fibronectinpurified from human plasma. Scanning electron microscopy (SEM) was used to study the morphological pattern of cell growth.