A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film
A simple device for multiplex ELISA made from melt-extruded plastic microcapillary film
复制标题
DOI：
10.1039/c0lc00357c
复制
发表时间：
2011-01-01
期刊：
影响因子：
6.1
通讯作者：
Mackley, Malcolm R.
中科院分区：
文献类型：
--
作者：
Edwards, Alexander D.;Reis, Nuno M.;Mackley, Malcolm R.
我们提出了一种简单的装置，用于多重定量酶联免疫吸附测定（ELISA）从一种新的熔融挤出微毛细管膜（MCF）含有平行阵列的200 μ m毛细管沿着其长度。为了制造ELISA装置，将不同的蛋白质抗原或抗体固定在从氟化乙烯丙烯（FEP）挤出的MCF的长卷轴内的各个微毛细管内。切下短片的涂布膜并与移液管连接，从而允许样品和检测溶液从试剂孔顺序摄取到所有毛细管中。这些FEP MCF装置不仅生产简单，而且具有优异的透光率，允许直接对毛细管进行光学询问，以进行简单的信号量化。概念验证实验证明了使用标准直接ELISA程序在FEP MCF装置中进行定量和多重测定，并使用平板扫描仪进行读取。这种新的多重免疫分析平台应该可以应用于从实验室检测到护理点和现场诊断的各种应用。
We present a simple device for multiplex quantitative enzyme-linked immunosorbant assays (ELISA) made from a novel melt-extruded microcapillary film (MCF) containing a parallel array of 200 mu m capillaries along its length. To make ELISA devices different protein antigens or antibodies were immobilised inside individual microcapillaries within long reels of MCF extruded from fluorinated ethylene propylene (FEP). Short pieces of coated film were cut and interfaced with a pipette, allowing sequential uptake of samples and detection solutions into all capillaries from a reagent well. As well as being simple to produce, these FEP MCF devices have excellent light transmittance allowing direct optical interrogation of the capillaries for simple signal quantification. Proof of concept experiments demonstrate both quantitative and multiplex assays in FEP MCF devices using a standard direct ELISA procedure and read using a flatbed scanner. This new multiplex immunoassay platform should find applications ranging from lab detection to point-of-care and field diagnostics.