Hepatotoxicity and metabolic effects of cellular extract of cyanobacterium Radiocystis fernandoi containing microcystins RR and YR on neotropical fish (Hoplias malabaricus).
Hepatotoxicity and metabolic effects of cellular extract of cyanobacterium Radiocystis fernandoi containing microcystins RR and YR on neotropical fish (Hoplias malabaricus).
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DOI：
10.1016/j.chemosphere.2017.02.089
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发表时间：
2017-05
期刊：
影响因子：
8.8
通讯作者：
M. G. Paulino;P. A. Rossi;F. P. Venturini;D. Tavares;Naiara Elisabete da Silva Souza;M. M. Sakuragui-M.;G. Moraes;A. Terezan;J. Fernandes;A. Giani;M. Fernandes
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文献类型：
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作者：
M. G. Paulino;P. A. Rossi;F. P. Venturini;D. Tavares;Naiara Elisabete da Silva Souza;M. M. Sakuragui-M.;G. Moraes;A. Terezan;J. Fernandes;A. Giani;M. Fernandes
本文研究了含有RR和YR微囊藻毒素的放射性藻R28细胞提取物对点带钩蚌（Hoplias malabaricus）的短期毒性效应，重点研究了其对肝脏结构和能量代谢的影响。将鱼腹膜内（i. p.）注射100 μg相当于MC-LR kg-1体重的费尔南多红球菌菌株R28细胞提取物。注射后12小时和96小时，采集血浆、肝脏和白色肌肉样本进行生化分析，还采集肝脏样本进行形态学分析。腹腔注射后，血浆中酸性磷酸酶（ACP）、丙氨酸氨基转移酶（ALT）和直接胆红素活性升高，而肝脏中ALT和天冬氨酸氨基转移酶（AST）活性降低。血浆中葡萄糖、乳酸和丙酮酸增加，而蛋白质减少;肝脏中糖原、丙酮酸和乳酸减少;肌肉中糖原和葡萄糖增加。血浆、肝脏和肌肉中的氨增加。细胞提取物注射后，肝细胞形状从多面体变为圆形;胆管组织丢失，但胆管形态在肝实质中保留。总之，微囊藻毒素对马拉巴河藻肝脏结构有一定影响，但肝脏能够通过调节中间代谢持续产生能量，糖原分解和糖原异生维持葡萄糖稳态和能量供应。
The toxicological effect of cellular extract of cyanobacteriumRadiocystis fernandoistrain R28 containing RR and YR microcystins was analyzed in the fishHoplias malabaricuswith emphasis on the liver structure and energetic metabolism, after short-term exposure. Fish were intraperitoneally (i.p.) injected with 100 μg of equivalent MC-LR kg−1body mass containing in the cellular extract ofR. fernandoistrain R28. Twelve and 96 h post-injection, the plasma, liver and white muscle were sampled for biochemical analyses and liver was also sampled for morphological analyses. After i.p. injection, the activity of acid phosphatase (ACP), alanine aminotransferase (ALT) and direct bilirubin increased in the plasma, while ALT and aspartate aminotransferase (AST) decreased in the liver. Glucose, lactate and pyruvate increased while protein decreased in the plasma; glycogen, pyruvate and lactate decreased in the liver; and glycogen and glucose increased in the muscle. Ammonia increased in the plasma, liver and muscle. The hepatocyte cell shape changed from polyhedral to round after cellular extract injection; there was loss of biliary canaliculus organization, but the biliary duct morphology was conserved in the liver parenchyma. In conclusion, microcystins present in the cellular extract ofR. fernandoistrain R28 affect the liver structure ofH. malabaricus, but the liver was able to continuously produce energy by adjusting its intermediate metabolism; glycogenolysis and gluconeogenesis maintained glucose homeostasis and energy supply.