F2-isoprostane and prostaglandin F2α metabolite excretion rate and day to day variation in healthy humans
F2-isoprostane and prostaglandin F2α metabolite excretion rate and day to day variation in healthy humans
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DOI：
10.1054/plef.2001.0295
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发表时间：
2001-08-01
影响因子：
3
通讯作者：
Basu, S
中科院分区：
文献类型：
--
作者：
Helmersson, J;Basu, S
异前列烷主要通过花生四烯酸的非酶自由基催化氧化在体内形成。研究表明，血浆和尿液中的主要异前列腺素8-iso-PGF（2 α）是氧化应激的可靠生物标志物。前列腺素是由环氧合酶（考克斯）催化的花生四烯酸的酶促氧化形成的。15-酮-二氢-PGF（2 α）是血浆中前列腺素F-2 α的主要代谢物，也存在于尿液中，被认为是炎症的有用生物标志物。为了研究8-iso-PGF（2 α）和15-keto-dihydro-PGF（2 α）在健康人中的排泄模式和日间变化，收集了13名志愿者连续10天的晨尿样品。通过放射免疫测定法分析样品的游离8-iso-PGF（2 α）和15-酮-二氢-PGF（2 α）。8-iso-PGF（2 α）的平均排泄率为0.27 +/-0.11 nmol/mmol肌酐（平均值+/- SD，n=13），10天内的变异系数为42%。15-酮-二氢-PGF（2 α）的平均排泄率为0.46 +/-0.19 nmol/mmol肌酐，变异系数为41%。8-iso-PGF（2 α）与15-keto-dihydro-PGF（2 α）的平均值呈显著正相关（r=0.68，P=0.01）。总之，在评价临床研究时，应考虑8-iso-PGF（2 α）和15-keto-dihydro-PGF（2 α）尿排泄率的日常生物学变化，除非这些参数大幅增加或减少。(C)2001年哈考特出版社有限公司
Isoprostanes are mainly formed in vivo by a non-enzymatic free radical catalysed oxidation of arachidonic acid. Studies have indicated that a major isoprostane, 8-iso-PGF(2 alpha) in plasma and urine is a reliable biomarker of oxidative stress. Prostaglandins are formed by enzymatic oxidation of arachidonic acid catalysed by cyclooxygenase (COX). 15-Keto-dihydro-PGF(2 alpha), a major metabolite of prostaglandin F-2 alpha, in plasma, and also found in urine, is considered to be a useful biomarker of inflammation. To investigate the excretion pattern and day to day variation of 8-iso-PGF(2 alpha), and 15-keto-dihydro-PGF(2 alpha) in healthy individuals, morning urine samples were collected from 13 volunteers on 10 successive days. The samples were analysed for free 8-iso-PGF(2 alpha) and 15-keto-dihydro-PGF(2 alpha) by radioimmunoassay. The mean excretion rate of 8-iso-PGF(2 alpha) was 0.27 +/-0.11 nmol/mmol creatinine (mean +/- SD, n=13) and the coefficient of variation was 42% during the 10 days. The mean excretion rate of 15-keto-dihydro-PGF(2 alpha) was 0.46 +/-0.19 nmol/mmol creatinine, giving a coefficient of variation of 41%. The mean values of 8-iso-PGF(2 alpha) were significantly correlated with the mean values of 15-keto-dihydro-PGF(2 alpha) (r=0.68, P=0.01). In conclusion, day to day biological variation in urinary excretion rate of 8-iso-PGF(2 alpha) and 15-keto-dihydro-PGF(2 alpha) should be taken into account in evaluating a clinical study unless a large increase or decrease of these parameters has been obtained. (C) 2001 Harcourt Publishers Ltd.