Long-Term In Vitro Culture of the Syphilis Spirochete Treponema pallidum subsp. pallidum.
Long-Term In Vitro Culture of the Syphilis Spirochete Treponema pallidum subsp. pallidum.
复制标题
DOI：
10.1128/mbio.01153-18
复制
发表时间：
2018-06-26
期刊：
影响因子：
6.4
通讯作者：
Norris SJ
中科院分区：
文献类型：
--
作者：
Edmondson DG;Hu B;Norris SJ
梅毒螺旋体亚种调查。尽管一个多世纪的努力，但由于无法在体外连续培养梅毒螺旋体，梅毒螺旋体一直受到阻碍。在这项研究中，通过每6至7天进行继代培养，并使用改良培养基（T. pallidum培养基2 [TpCM-2]）与先前描述的微氧兔上皮细胞共孵育系统进行周期性饲养，实现了T. pallidum的长期对数增殖。目前，培养物已保持连续生长超过6个月，通过运动性和兔传染性来测量其完全保留活力。该系统已成功应用于研究充分的梅毒T. Nichols菌株，以及最近分离的两株梅毒，UW231B和UW249B。光镜和冷冻电镜观察表明，体外培养的苍白球菌保持野生型形态。这一长期传代培养系统的进一步完善有望促进白衣绦虫亚种生理、遗传、病理、免疫和抗菌敏感性的研究。梅毒螺旋体和密切相关的致病性螺旋体种和亚种。梅毒是一种全球性的性传播疾病，由一种名为梅毒螺旋体亚种梅毒螺旋体的螺旋状细菌引起。在此之前，T. pallidum是少数几个没有在体外（试管中）长期培养的主要细菌病原体之一，这极大地阻碍了人们更好地了解这种有机体及其引起的疾病。在这篇文章中，我们报道了在组织培养系统中成功的长期培养梅毒T. pallidum，这一发现可能会增强我们获得适用于梅毒诊断、治疗和预防的新信息的能力。
Investigation of Treponema pallidum subsp. pallidum, the spirochete that causes syphilis, has been hindered by an inability to culture the organism continuously in vitro despite more than a century of effort. In this study, long-term logarithmic multiplication of T. pallidum was attained through subculture every 6 to 7 days and periodic feeding using a modified medium (T. pallidum culture medium 2 [TpCM-2]) with a previously described microaerobic, rabbit epithelial cell coincubation system. Currently, cultures have maintained continuous growth for over 6 months with full retention of viability as measured by motility and rabbit infectivity. This system has been applied successfully to the well-studied Nichols strain of T. pallidum, as well as to two recent syphilis isolates, UW231B and UW249B. Light microscopy and cryo-electron microscopy showed that in vitro-cultured T. pallidum retains wild-type morphology. Further refinement of this long-term subculture system is expected to facilitate study of the physiological, genetic, pathological, immunologic, and antimicrobial susceptibility properties of T. pallidum subsp. pallidum and closely related pathogenic Treponema species and subspecies. Syphilis, a sexually transmitted disease with a global distribution, is caused by a spiral-shaped bacterium called Treponema pallidum subspecies pallidum. Previously, T. pallidum was one of the few major bacterial pathogens that had not been cultured long-term in vitro (in a test tube), greatly hindering efforts to better understand this organism and the disease that it causes. In this article, we report the successful long-term cultivation of T. pallidum in a tissue culture system, a finding that is likely to enhance our ability to obtain new information applicable to the diagnosis, treatment, and prevention of syphilis.