Virus-Induced LeSPL-CNR Silencing Inhibits Fruit Ripening in Tomato
Virus-Induced LeSPL-CNR Silencing Inhibits Fruit Ripening in Tomato
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病毒诱导的 LeSPL-CNR 沉默抑制番茄果实成熟
DOI：
10.5539/jas.v7n7p184
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发表时间：
2015
影响因子：
2
通讯作者：
洪益国
中科院分区：
文献类型：
--
作者：
赖童飞;汪莹;周婷;梅凤玲;章鹏程;周莹莹;施农农;洪益国
果实成熟是一个发育和遗传程序化的过程。在番茄（Solanum lycopersicum）中，成熟决定了果实品质、商品价值、货架期和许多重要属性。为了理解这一复杂的过程及其基础机制，需要一种高效和有效的方法来筛选和功能分析成熟相关基因（RAGs）。病毒诱导的基因沉默（VIGS）是揭示植物基因功能的一种强有力的反向遗传学工具。VIGS已被用来研究RAGs在番茄成熟中的作用。然而，在大多数情况下，病毒诱导的RAG沉默仅被评估并与果实的颜色变化相关。在这里，我们报道了通过基于马铃薯X病毒的VIGS沉默LeSPL-CNR抑制了果实成熟并导致Ailsa克雷格（AC）番茄中非成熟区的发育。未成熟部分的硬度较高，相对电导率和酸度较大，叶绿素含量较高，但花青素含量较低。LeSPL-CNR的VIGS还影响其他关键RAG和与成熟激素乙烯的生物发生相关的基因的表达。这些结果表明，经历LeSPL-CNR的VIGS的AC果实表型模仿无色不熟表型突变体的物理、生理、农业化学、生化和分子特征。因此，LeSPL-CNR沉默引起的从视觉外观到RAG表达的整体表型变化再次证实了VIGS在揭示番茄成熟和果实品质中至关重要的基因的生物学功能方面的巨大用途。
Fruit ripening is a developmentally and genetically programmed process. In tomato ( Solanum lycopersicum ), ripening determines fruit quality, commodity value, shelf life and many important attributes. To understand this intricate process and its underpinning mechanism, an efficient and effective approach for screening and functional analysis of ripening-associated genes (RAGs) is required. Virus-induced gene silencing (VIGS) is a powerful reverse genetics tool for uncovering gene functions in plants. VIGS has been exploited to investigate roles of RAGs in tomato ripening. However in most cases, virus-induced RAG silencing is only assessed and correlated with the chromatic change of fruits. Here we report that silencing of LeSPL-CNR through a Potato virus X -based VIGS inhibited fruit ripening and led to development of non-ripening sectors in Ailsa Craig (AC) tomatoes. Non-ripening sectors remained firmer and possessed greater relative electric conductivity and acidity as well as a higher amount of chlorophyll, but a lower quantity of anthocyanin. VIGS of LeSPL-CNR also affects expression of other key RAGs and genes associated with biogenesis of ripening hormone ethylene. These findings indicate that AC fruits undergoing VIGS of LeSPL-CNR phenocopied physical, physiological, agrochemical, biochemical and molecular characteristics of the Colourless non-ripening epimutant. Thus, the overall phenotypical changes from visual appearance to RAG expression caused by LeSPL-CNR silencing reaffirm the great usefulness of VIGS to reveal biological functions of genes crucial in tomato ripening and fruit quality.