Phylogenetic congruence and ecological coherence in terrestrial Thaumarchaeota.
Phylogenetic congruence and ecological coherence in terrestrial Thaumarchaeota.
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DOI：
10.1038/ismej.2015.101
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Gubry-Rangin C
中科院分区：
文献类型：
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作者：
Oton EV;Quince C;Nicol GW;Prosser JI;Gubry-Rangin C
奇古菌门是一个分布广泛的古菌门，包括氨氧化古菌（AOA）和其他尚未证实氨氧化的古菌群（包括1.1c组和1.3组）。陆地环境中 AOA 的生态学已通过编码氨单加氧酶亚基 A (amoA) 的功能基因或 16S 核糖体 RNA (rRNA) 基因进行了广泛研究，这些基因显示出与土壤 pH 相关的系统发育一致性。为了测试这两个标记之间的系统发育一致性并确定所有奇古菌的生态一致性，我们对 46 个英国土壤中的 16S rRNA 和 amoA 基因进行了高通量测序，呈现了 29 种可用的背景土壤特征。对 pH 值和有机质含量的适应反映了奇古菌（AOA 和非 AOA）在不同分类分辨率水平上的强烈生态一致性，而氮、总矿化氮和锌浓度也是与 AOA 奇古菌群落分布相关的重要因素。还检测到 amoA 和 16S rRNA 基因与环境因素的其他显着关联，反映了这两个标记之间不同的多样性特征。尽管如此，在精细系统发育分辨率下，标记之间存在显着的统计一致性，支持奇古菌群之间水平基因转移水平较低的假设。 1.1c 组奇古菌也分布广泛，以两个簇为主，特别是在水分含量和有机质较高的环境中，而 1.3 组奇古菌也观察到类似的生态模式。所确定的生态和系统发育一致性对于更好地了解奇古菌的生命策略、进化历史和生态系统功能至关重要。
Thaumarchaeota form a ubiquitously distributed archaeal phylum, comprising both the ammonia-oxidising archaea (AOA) and other archaeal groups in which ammonia oxidation has not been demonstrated (including Group 1.1c and Group 1.3). The ecology of AOA in terrestrial environments has been extensively studied using either a functional gene, encoding ammonia monooxygenase subunit A (amoA) or 16S ribosomal RNA (rRNA) genes, which show phylogenetic coherence with respect to soil pH. To test phylogenetic congruence between these two markers and to determine ecological coherence in all Thaumarchaeota, we performed high-throughput sequencing of 16S rRNA and amoA genes in 46 UK soils presenting 29 available contextual soil characteristics. Adaptation to pH and organic matter content reflected strong ecological coherence at various levels of taxonomic resolution for Thaumarchaeota (AOA and non-AOA), whereas nitrogen, total mineralisable nitrogen and zinc concentration were also important factors associated with AOA thaumarchaeotal community distribution. Other significant associations with environmental factors were also detected for amoA and 16S rRNA genes, reflecting different diversity characteristics between these two markers. Nonetheless, there was significant statistical congruence between the markers at fine phylogenetic resolution, supporting the hypothesis of low horizontal gene transfer between Thaumarchaeota. Group 1.1c Thaumarchaeota were also widely distributed, with two clusters predominating, particularly in environments with higher moisture content and organic matter, whereas a similar ecological pattern was observed for Group 1.3 Thaumarchaeota. The ecological and phylogenetic congruence identified is fundamental to understand better the life strategies, evolutionary history and ecosystem function of the Thaumarchaeota.