Metabolites in the root exudates of groundnut change during interaction with plant growth promoting rhizobacteria in a strain-specific manner
Metabolites in the root exudates of groundnut change during interaction with plant growth promoting rhizobacteria in a strain-specific manner
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DOI：
10.1016/j.jplph.2019.153057
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发表时间：
2019-12-01
影响因子：
4.3
通讯作者：
Podile, Appa Rao
中科院分区：
文献类型：
--
作者：
Ankati, Sravani;Podile, Appa Rao
植物根际促生菌（PGPR）作为生物肥料被广泛应用于各种作物的土壤培肥。与其他有益的植物-微生物相互作用不同，植物-PGPR相互作用，特别是化学信号分子的相互作用还不清楚。PGPR从土壤微生物库向植物的化学吸引力可能依赖于植物根系分泌物（RE）中的一些分子，类似于豆类根瘤菌的有益关联。在这项研究中，PGPR的一些功能特性，如生长，趋化性和生物膜形成的两个PGPR菌株，即，芽孢杆菌sonorensis RS 4和铜绿假单胞菌RP 2进行了评估，在花生REs的存在下。这两种菌株的功能特性显着影响的REs在一个菌株依赖的方式。生长12天和24天后，用GC-MS/MS对PGPR细菌化（RS 4或RP 2）和非细菌化幼苗的RE进行代谢产物分析。共检测到75种代谢产物，在花生REs。苏氨酸和乙醛肟酸检测到RP 2细菌化的REs，而丝氨酸，戊酸，葡萄糖苷，酒石酸，2-吡咯烷酮检测到与RP 2和RS 4细菌化的幼苗REs。结果表明，PGPR诱导了RE的明显变化，鉴定PGPR互作特异性代谢产物将有助于开发有效的PGPR生物制剂，以提高PGPR定殖效果和提高作物产量。
Plant growth promoting rhizobacteria (PGPR) are extensively used as biofertilizers to improve the soil nutrition for a variety of crop plants. The plant-PGPR interaction, with special reference to chemical signalling molecules is not understood clearly, unlike other beneficial plant-microbe interactions. Chemo-attraction of a PGPR from soil microbial pool towards a plant could be dependent on some of the molecules in the plant root exudates (REs), similar to the beneficial association of legume-rhizobia. In this study, a few functional properties of PGPR like growth, chemotaxis, and biofilm formation by two PGPR strains viz., Bacillus sonorensis RS4 and Pseudomonas aeruginosa RP2 were assessed in the presence of groundnut REs. Functional properties of both the strains were significantly influenced by the REs in a strain-dependent manner. Metabolite profiling of the REs from PGPR-bacterized (RS4 or RP2) and non-bacterized seedlings was performed with GC-MS/MS after 12 and 24 days of growth. A total of 75 metabolites were detected in groundnut REs. Threonine and glyoxylic oxime acid were detected in RP2-bacterized REs, while serine, pentanoic acid, glucopyranoside, tartaric acid, and 2-pyrrolidinone were detected in REs of seedlings bacterized with RP2 and RS4. The results suggested that the PGPR induced distinct variations in the REs. Identification of the interaction-specific metabolites will be useful to develop effective PGPR based bio-formulations for better PGPR colonization and improving crop yields.