REGULATION OF BENZOATE-COA LIGASE IN RHODOPSEUDOMONAS-PALUSTRIS
REGULATION OF BENZOATE-COA LIGASE IN RHODOPSEUDOMONAS-PALUSTRIS
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DOI：
10.1016/0378-1097(91)90354-d
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发表时间：
1991-10-01
影响因子：
2.1
通讯作者：
HARWOOD, CS
中科院分区：
文献类型：
--
作者：
KIM, MK;HARWOOD, CS
沼泽红球藻厌氧降解苯甲酸的第一步是由苯甲酸辅酶A连接酶催化的。为了研究影响该酶合成的因素，制备了多克隆抗血清并用于免疫印迹测定。当细胞与苯甲酸盐以及与羟基和甲基取代的苯甲酸盐一起生长时，合成苯甲酸-CoA连接酶。部分减少脂环族化合物提出的苯甲酸途径中的中间体也诱导苯甲酸-CoA连接酶。连接酶的合成被氧抑制。诱导剂的多样性与苯甲酸酯是具有更复杂结构的各种芳香酸的降解中的中心中间体的观察结果一致。
The first step in the anaerobic pathway of benzoate degradation by Rhodopseudomonas palustris is catalyzed by benzoate-coenzyme A ligase. To study factors influencing the synthesis of this enzyme, a polyclonal antiserum was prepared and used in immunoblot assays. Benzoate-CoA ligase was synthesized when cells were grown with benzoate, as well as with hydroxyl- and methyl-substituted benzoates. Partially reduced alicyclic compounds proposed to be intermediates in the benzoate pathway also induced benzoate-CoA ligase. Ligase synthesis was repressed by oxygen. The diversity of inducers is consistent with the observation that benzoate is a central intermediate in the degradation of a variety of aromatic acids with more complex structures.