FLOURY ENDOSPERM19 encoding a class I glutamine amidotransferase affects grain quality in rice
FLOURY ENDOSPERM19 encoding a class I glutamine amidotransferase affects grain quality in rice
复制标题
编码 I 类谷氨酰胺酰胺转移酶的 FLOURY ENDOSPERM19 影响稻米品质
DOI：
10.1007/s11032-021-01226-z
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发表时间：
2021-06-01
影响因子：
3.1
通讯作者：
He, Yuqing
中科院分区：
文献类型：
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作者：
Lou, Guangming;Chen, Pingli;He, Yuqing
作为世界一半以上人口的主食，大米的重要性不言而喻。与普通大米相比，食味品质和外观品质优越的水稻品种，由于其独特的口感和观赏价值，即使价格要高很多，也更受消费者的欢迎。外观品质和CEQ（蒸煮食味品质）是评价大米品质的两个非常重要的方面。在这里，我们对 533 个栽培稻种质的不同组中的粉质胚乳进行了全基因组关联研究。我们鉴定了一批潜在的面粉基因，并优先考虑其中一个 (LOC_Os03g48060) 进行功能分析。通过编辑LOC_Os03g48060（本研究中命名为FLO19）产生了两个粉质外胚乳突变体（flo19-1和flo19-2），它编码I类谷氨酰胺酰胺转移酶。两个突变体在各种储存物质中的不同表现直接导致了CEQ完全不同的变化。 FLO19基因突变导致水稻碳氮代谢受损，影响水稻正常生长发育，表现为株高降低，灌浆率降低导致产量下降。通过单倍型分析，我们鉴定出FLO19的一个同时提高水稻CEQ和外观品质的单倍型Hap2，为水稻品质改良，特别是高产籼稻品种提供了选择靶标。
As a staple food for more than half of the world's population, the importance of rice is self-evident. Compared with ordinary rice, rice cultivars with superior eating quality and appearance quality are more popular with consumers due to their unique taste and ornamental value, even if their price is much higher. Appearance quality and CEQ (cooking and eating quality) are two very important aspects in the evaluation of rice quality. Here, we performed a genome-wide association study on floury endosperm in a diverse panel of 533 cultivated rice accessions. We identified a batch of potential floury genes and prioritize one (LOC_Os03g48060) for functional analyses. Two floury outer endosperm mutants (flo19-1 and flo19-2) were generated through editing LOC_Os03g48060 (named as FLO19 in this study), which encodes a class I glutamine amidotransferase. The different performances of the two mutants in various storage substances directly led to completely different changes in CEQ. The mutation of FLO19 gene caused the damage of carbon and nitrogen metabolism in rice, which affected the normal growth and development of rice, including decreased plant height and yield loss by decreased grain filling rate. Through haplotype analysis, we identified a haplotype of FLO19 that can improve both CEQ and appearance quality of rice, Hap2, which provides a selection target for rice quality improvement, especially for high-yield indica rice varieties.