Evaluation of Liver Preservation Solutions by Using Rats Transgenic for Luciferase
Evaluation of Liver Preservation Solutions by Using Rats Transgenic for Luciferase
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DOI：
10.1016/j.transproceed.2013.07.077
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发表时间：
2014-01-01
影响因子：
0.9
通讯作者：
Kobayashi, E.
中科院分区：
文献类型：
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作者：
Doi, J.;Teratani, T.;Kobayashi, E.
导论.保存移植组织的溶液（即保存液）是肝移植技术的重要组成部分。其保护作用是由溶液中的物质诱导的，包括自由基清除剂、缓冲剂和能量给予物质。新的保存液已被证明能有效防止冷缺血时器官损伤并延长保存时限。本研究旨在探讨荧光素酶荧光强度与肝组织中三磷酸腺苷（ATP）含量之间的关系，并提出一种新的离体筛选系统，该系统使用转荧光素酶基因的刘易斯大鼠来评价保存液的有效性。从转基因大鼠获得肝脏样品（直径，2 mm）。通过测定ATP含量和发光强度，对这些组织在威斯康星州（UW）溶液、京都的胞外海藻糖溶液、Euro-Collins（EC）溶液、组氨酸-色氨酸-酮氟二酸盐溶液、低钾右旋糖酐溶液或生理盐水中储存长达6 h后的活力进行评估。发光强度与ATP浓度呈线性关系（R = 0.88）。无论使用哪种保存溶液，肝组织芯片的发光强度随时间线性降低，特别是在短时间内（0至2小时; R-2 = 0.58-1.0）。在UW液中保存2 ~ 6 h的肝芯片组织的发光强度明显高于其他保存液（P <0.05; 6 h）。根据发光强度，EC可能优于用于超短期储存（0.5至2小时）的其他测试溶液。我们的模型结合了生物成像系统和荧光素酶转基因刘易斯大鼠的使用，有效地评估了肝组织样品的活力。我们相信，这种离体筛选系统将是一个有效的工具，用于评估保存解决方案的肝移植。
Introduction. The solution in which graft tissue is stored (that is, preservation solution) is an important component of liver transplantation technology. Its protective effect is induced by substances in the solution, including radical scavengers, buffers, and energy-giving substances. New preservation solutions have proven to be effective in preventing organ damage during cold ischemia and in extending the time limits for storage.Aim. This study determined the relationship between luminescence intensity and content of adenosine triphosphate (ATP) in liver tissue and proposes a new ex vivo screening system that uses Lewis rats transgenic for luciferase for evaluating the effectiveness of preservation solutions.Methods. Samples (diameter, 2 mm) of liver were obtained from transgenic rats. The viability of these tissues after storage for as long as 6 hours in University of Wisconsin (UW) solution, extracellular trehalose solution of Kyoto, Euro-Collins (EC) solution, histidine-tryptophan-ketoflutarate solution, low potassium dextran solution, or normal saline was assessed by determining ATP content and luminescence intensity.Results. Luminescence had a linear relationship (R = 0.88) with ATP levels. Regardless of the preservation solution used, the luminescence intensities of the liver tissue chips decreased linearly with time especially through a short span of time (0 to 2 hours; R-2 = 0.58-1.0). The luminescence of liver chip tissues maintained long term (2 to 6 hours) in UW solution tended to be higher than those of tissues stored in other solutions (P < .05; 6 hours). On the basis of luminescence intensity, EC might be preferable to the other solutions tested for ultra-short term storage (0.5 to 2 hours).Conclusion. Our model, which combines the use of the bioimaging system and Lewis rats transgenic for luciferase, effectively assessed the viability of liver tissue samples. We believe that this ex vivo screening system will be an effective tool for evaluating preservation solutions for liver grafts.