Bacterial and fungal communities of wilted Italian ryegrass silage inoculated with and without Lactobacillus rhamnosus or Lactobacillus buchneri
Bacterial and fungal communities of wilted Italian ryegrass silage inoculated with and without Lactobacillus rhamnosus or Lactobacillus buchneri
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DOI：
10.1111/j.1472-765x.2010.03000.x
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发表时间：
2011-04-01
影响因子：
2.4
通讯作者：
Nishino, N.
中科院分区：
文献类型：
--
作者：
Li, Y.;Nishino, N.
目的：了解乳酸菌（LAB）接种对青贮料发酵产物、好氧稳定性和微生物群落的影响。方法和结果：将多花黑麦草在实验室青贮窖中分别接种和不接种鼠李糖乳杆菌（Lactobacillus rhamnosus）和布氏乳杆菌（Lactobacillus buchneri）。筒仓在14、56和120天后打开，然后进行有氧变质7天。在未处理的青贮饲料中发现了强烈的酒精发酵;在第14天乙醇和2，3-丁二醇含量的总和比乳酸和挥发性脂肪酸高约7倍。L.鼠李糖乳杆菌L. Buchneri接种以及乳酸和乙酸分别成为主要的发酵产物。未处理和L.接种鼠李糖的青贮饲料，而L.布氏接种的青贮饲料。在未处理的青贮饲料中检测到肠杆菌，如欧文氏菌、成团泛菌和水生拉恩氏菌，而在L. rhamnosus治疗。当青贮饲料变质时，未处理和L.鼠李糖接种的社区，分别。除了未处理的细菌群落之外，接种的LAB物种是可检测的。酿酒酵母和异常毕赤酵母是未处理和L.接种鼠李糖的青贮饲料;然而，在L.结论：接种L.鼠李糖酵母能抑制萎蔫草青贮的酒精发酵，并在发酵开始时消除肠杆菌。添加L。Buchneri可以提高好氧稳定性，与观察到的P. anomala打开筒仓后的显著抑制效果。研究的意义和影响：细菌和真菌群落分析有助于我们了解接种LAB如何能够发挥作用，以提高青贮饲料的发酵和好氧稳定性。
Aims:To understand the effects of lactic acid bacteria (LAB) inoculation on fermentation products, aerobic stability and microbial communities of silage.Methods and Results:Wilted Italian ryegrass was stored in laboratory silos with and without inoculation of Lactobacillus rhamnosus and Lactobacillus buchneri. The silos were opened after 14, 56 and 120 days and then subjected to aerobic deterioration for 7 days. Intensive alcoholic fermentation was found in untreated silage; the sum of ethanol and 2,3-butanediol content at day 14 was about 7 times higher than that of lactic and volatile fatty acids. Alcoholic fermentation was suppressed by L. rhamnosus and L. buchneri inoculation and lactic acid and acetic acid became the dominant fermentation products, respectively. Silages were deteriorated in untreated and L. rhamnosus-inoculated silages, whereas no spoilage was found in L. buchneri-inoculated silage. Enterobacteria such as Erwinia persicina, Pantoea agglomerans and Rahnella aquatilis were detected in untreated silage, whereas some of these bacteria disappeared or became faint with L. rhamnosus treatment. When silage was deteriorated, Lactobacillus brevis and Bacillus pumilus were observed in untreated and L. rhamnosus-inoculated communities, respectively. The inoculated LAB species was detectable in addition to untreated bacterial communities. Saccharomyces cerevisiae and Pichia anomala were the main fungi in untreated and L. rhamnosus-inoculated silages; however, P. anomala was not visibly seen in L. buchneri-inoculated silage either at silo opening or after exposure to air.Conclusion:Inoculation with L. rhamnosus can suppress alcoholic fermentation of wilted grass silage with elimination of enterobacteria at the beginning of fermentation. Addition of L. buchneri may improve aerobic stability, with distinct inhibitory effect observed on P. anomala after silo opening.Significance and Impact of the Study:Bacterial and fungal community analyses help us to understand how inoculated LAB can function to improve the fermentation and aerobic stability of silage.