Expression of mRNAs and proteins for peroxiredoxins in Plasmodium falciparum erythrocytic stage
Expression of mRNAs and proteins for peroxiredoxins in Plasmodium falciparum erythrocytic stage
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DOI：
10.1016/j.parint.2004.08.005
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发表时间：
2005-03-01
影响因子：
1.9
通讯作者：
Kawazu, S
中科院分区：
文献类型：
--
作者：
Yano, K;Komaki-Yasuda, K;Kawazu, S
采用实时定量逆转录pcr （RT-PCR）、Western blot和共聚焦激光扫描显微镜检测恶性疟原虫红细胞期3种过氧化物还毒素（PfFPx-1、PfrPx-2和Pfl-Cys-Prx）和1种硫氧还毒素（PfFrx-1） mRNA和蛋白表达谱。PfTPx-1在整个红细胞阶段都在寄生虫的细胞质中组成性表达，这表明该酶在控制寄生虫的细胞间活性氧（ROS）方面起着管家作用。Pfl-Cys-Prx在寄生虫细胞质的滋养体和早期分裂体阶段表达升高，这表明这种过氧化物还毒素（Prx）可以解毒代谢产生的活性氧，如血红素铁释放的活性氧。另一个2-Cys Prx， PfFPx-2，在线粒体中检测到，在滋养体和分裂体阶段都表达。在线粒体中检测到Prx与最近报道的恶性疟原虫线粒体中存在产生ROS的呼吸链相一致。PfTrx-1在寄生细胞质滋养体和分裂体阶段表达升高。最后，这些抗氧化蛋白基因的表达很可能在转录水平上受到调控，因为它们的mRNA和蛋白质表达谱重叠。2004爱思唯尔爱尔兰有限公司版权所有。
mRNA and protein expression profiles for three peroxiredoxins (PfFPx-1, PfrPx-2 and Pfl-Cys-Prx) and a thioredoxin (PfFrx-1) of Plasmodium falciparum during the erythrocytic stage were examined by real-time quantitative reverse transcription-PCR (RT-PCR), Western blotting and confocal laser scanning microscopy. PfTPx-1 was expressed constitutively in the parasite cytoplasm throughout the erythrocytic stage, suggesting a housekeeping role of this enzyme for control of intercellular reactive oxygen species (ROS) in the parasite. Pfl-Cys-Prx showed elevated expression during the trophozoite and early schizont stages in the parasite cytoplasm, and this profile suggested that this peroxiredoxin (Prx) detoxifies metabolism-derived ROS such as those released from heme iron. The other 2-Cys Prx, PfFPx-2, was detected in mitochondria and was expressed in both the trophozoite and schizont stages. Detection of the Prx in mitochondria is consistent with recent reports of the existence of a respiratory chain, which produces ROS, in the mitochondria of P. falciparum. PfTrx-1 showed elevated expression during the trophozoite and schizont stages in the parasite cytoplasm. Finally, expression of these antioxidant protein genes is most likely regulated at the transcriptional level because their mRNA and protein expression profiles overlapped. (C) 2004 Elsevier Ireland Ltd. All rights reserved.