Homogeneity of the Vaginal Microbiome at the Cervix, Posterior Fornix, and Vaginal Canal in Pregnant Chinese Women
Homogeneity of the Vaginal Microbiome at the Cervix, Posterior Fornix, and Vaginal Canal in Pregnant Chinese Women
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中国孕妇子宫颈、后穹窿和阴道管阴道微生物组的同质性
DOI：
10.1007/s00248-014-0487-1
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发表时间：
2015-02-01
影响因子：
3.6
通讯作者：
Zhou, Hong-Wei
中科院分区：
文献类型：
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作者：
Huang, Yi-E;Wang, Yan;Zhou, Hong-Wei
阴道微生物群是产前健康中一个新出现的问题。由于阴道微生物区系的采样过程可能会给孕妇带来潜在的风险，因此采样地点的选择应慎重考虑。然而，不同采样点的微生物多样性是否不同一直存在争议。本研究从34例不同孕期的中国妇女的宫颈(C)、后穹隆(P)和阴道(V)采集了3个重复拭子，并用16S rRNA标签序列的Illumina测序法对阴道菌种进行了鉴定。已鉴定的微生物群落可分为四种群落状态类型：Cst I(优势BYL)。Crispatus)、Cst II(优势种Byl.Asseri)、Cst III(优势种BYL.Iners)和CST IV-A(特征是乳杆菌丰度低，但之前显示与细菌性阴道病有关的各种物种的比例)。所有个体在三个采样点都有一致的CST，而不受妊娠阶段和CST组的影响。此外，根据左撇子的测定，每个个体内的群落结构之间几乎没有异质性，这表明三个采样点的阴道微生物群具有很高的同质性。本研究还揭示了怀孕阶段不同的β多样性。根据UniFrac距离(P<； )，T1孕期妇女的阴道微生物群变异(9 ± 2.6w)大于非妊娠妇女和其他妊娠妇女(P<； 0.05)。特别是，本研究首次证明产后妇女与妊娠期妇女的阴道微生物群有显著差异。这些结果将有助于未来对怀孕期间阴道微生物区系的研究。
The vaginal microbiome is an emerging concern in prenatal health. Because the sampling process of vaginal microbiota may pose potential risks for pregnant women, the choice of sampling site should be carefully considered. However, whether the microbial diversity is different across various sampling sites has been controversial. In the present study, three repeated swabs were collected at the cervix (C), posterior fornix (P), and vaginal canal (V) from 34 Chinese women during different pregnancy stages, and vaginal species were determined using the Illumina sequencing of 16S rRNA tag sequences. The identified microbiomes were classified into four community state types (CSTs): CST I (dominated byL. crispatus), CST II (dominated byL. gasseri), CST III (dominated byL. iners), and CST IV-A (characterized by a low abundance ofLactobacillus, but with proportions of various species previously shown to be associated with bacterial vaginosis). All individuals had consistent CST at the three sampling sites regardless of pregnancy stage and CST group. In addition, there was little heterogeneity across community structures within each individual, as determined by LEfSe, indicating high vaginal microbiome homogeneity at the three sampling sites. The present study also revealed different beta diversity during pregnancy stages. The vaginal microbiome variation among women during trimester T1 (9 ± 2.6 weeks) is larger than that of non-pregnant women and women from other trimesters, as demonstrated by the UniFrac distance (P< 0.05). In particular, the present study is the first one that demonstrates the notably difference of vaginal microbiome of postpartum women compare to women in gestation. These results will be useful for future studies of the vaginal microbiota during pregnancy.