Acinetobacter lwoffli and Lactococcus lactis strains isolated from farm cowsheds possess strong allergy-protective properties
Acinetobacter lwoffli and Lactococcus lactis strains isolated from farm cowsheds possess strong allergy-protective properties
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DOI：
10.1016/j.jaci.2007.03.023
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发表时间：
2007-06-01
影响因子：
14.2
通讯作者：
Heine, Holger
中科院分区：
文献类型：
--
作者：
Debarry, Jennifer;Garn, Holger;Heine, Holger
背景资料：在农业环境中长大的儿童比没有生活在这种环境中的同龄儿童表现出更低的特应性致敏、花粉症和哮喘水平。一些调查提供了很好的证据，表明这是由于早期与牛棚，农场动物和/或消费生奶等产品的接触。此外，微生物可能对变态反应的发展具有重要影响，因此出现了以下问题：哪些农场微生物、其产品或两者可能诱导或影响变态反应保护机制。目的：我们试图进一步了解从农场环境中分离的微生物的潜在变态反应保护特性。在农场的牛棚中鉴定的许多细菌物种中，选择、分离和表征了2种，即鲁氏不动杆菌F78和乳酸乳球菌G121。研究了这些分离株的模式识别受体激活、人单核细胞来源的树突状细胞的成熟、炎性细胞因子的上调、T（H）1极化Notch配体表达及其对过敏表型的影响。结果如下：结果表明，这两种细菌分离物都能够减少小鼠的过敏反应，在体外激活哺乳动物细胞，并诱导树突状细胞中的TH 1极化程序。结论：我们的数据强烈支持卫生假说，即富含微生物结构的环境，如农业环境，可能会防止过敏的发展。临床意义：这项工作提供了关于牛棚细菌在过敏保护中的潜在应用的第一个数据。
Background: Children who grow up in a farming environment show lower levels of atopic sensitization, hay fever, and asthma than children of the same age not living in such an environment. A number of investigations provided good evidence that this is due to an early-life contact with cowsheds, farm animals, and/or consumption of products like raw milk. Also, it had been indicated that microorganisms might have an important effect on the development of allergies, and thus the question arose of which farm microbial organisms, their products, or both might induce or influence allergy-protective mechanisms.Objective: We sought to gain further insight into the potential allergy-protective properties of microbes isolated from the farming environment.Methods: Of a number of bacterial species identified in cowsheds of farms, 2 were selected, isolated, and characterized, namely Acinetobacter lwoffii F78 and Lactococcus lactis G121. The isolates were investigated with regard to their activation of pattern-recognition receptors, the maturation of human monocyte-derived dendritic cells, the upregulation of inflammatory cytokines, the T(H)1-polarizing Notch ligand expression, and their influence on the allergic phenotype. Results: It is shown that both bacterial isolates were able to reduce allergic reactions in mice, to activate mammalian cells in vitro, and to induce a TH1-polarizing program in dendritic cells.Conclusion: Our data strongly support the hygiene hypothesis, which states that an environment rich in microbiologic structures, such as a farming environment, might protect against the development of allergies.Clinical implications: This work provides the first data on a potential application of cowshed bacteria in allergy protection.