Sertoli and granulosa cell-specific Cre recombinase activity in transgenic mice
Sertoli and granulosa cell-specific Cre recombinase activity in transgenic mice
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DOI：
10.1002/gene.10100
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发表时间：
2002-07-01
期刊：
影响因子：
1.5
通讯作者：
Guillou, F
中科院分区：
文献类型：
--
作者：
Lécureuil, C;Fontaine, I;Guillou, F
我们建立了在抗苗勒氏激素(AMH)基因启动子控制下表达Cre重组酶的转基因小鼠。用不同的方法评价Cre活性和特异性。在AMH-Cre小鼠中，Cre重组酶基因的表达仅限于睾丸和卵巢。AMH-Cre小鼠与报告转基因品系杂交，其后代仅在睾丸和卵巢表现出Cre介导的重组。在雄性，组织化学分析显示每个支持细胞都发生了重组。在雌性，Cre介导的重组仅限于颗粒细胞，但该蛋白在每个细胞中的活性并不均匀。根据这些结果，我们认为，该转基因株具有AMH启动子驱动的Cre重组酶的表达，可能是一种仅删除Sertoli细胞中基因的有效工具，以便研究哺乳动物精子发生过程中Sertoli细胞的基因功能。(C)2002年Wiley-Liss，Inc.
We have established transgenic mice expressing the Cre recombinase under the control of the anti-Mullerian hormone (AMH) gene promoter. Cre activity and specificity were evaluated by different means. In AMH-Cre mice, expression of the Cre recombinase mRNA was confined to the testis and ovary. AMH-Cre mice were crossed with reporter transgenic lines and the offspring exhibited Cre-mediated recombination only in the testis and the ovary. In male, histochemical analysis indicated that recombination occured in every Sertoli cells. In female, Cre-mediated recombination was restricted to granulosa cells, but the protein was not evenly active in every cells. From these results, we conclude that potentially, this transgenic line possessing AMH promoter-driven expression of the Cre recombinase is a powerful tool to delete genes in Sertoli cells only, in order to study Sertoli cell gene function during mammalian spermatogenesis. (C) 2002 Wiley-Liss, Inc.