Method for trapping and manipulating nanoscale objects in solution
Method for trapping and manipulating nanoscale objects in solution
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DOI：
10.1063/1.1872220
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发表时间：
2005-02-28
影响因子：
4
通讯作者：
Moerner, WE
中科院分区：
文献类型：
--
作者：
Cohen, AE;Moerner, WE
我们提出了一种设备，允许用户在环境温度下捕获溶液中的单个纳米级物体，然后以纳米级分辨率定位捕获的物体。这种反布朗电泳陷阱（ABEL 陷阱）的工作原理是监测粒子的布朗运动（通过荧光显微镜），然后向溶液施加反馈电压，使电泳漂移完全抵消布朗运动。 ABEL 陷阱适用于任何可以光学成像并在水中获取电荷的物体。 ABEL 陷阱是非侵入性的，足够温和，可以处理生物分子，并且可以捕获比激光镊子捕获的物体小得多的物体。我们的原理验证装置可以捕获直径小至 20 nm 的荧光聚苯乙烯纳米球。 (C) 2005 年美国物理研究所。
We present a device that allows a user to trap a single nanoscale object in solution at ambient temperature, and then to position the trapped object with nanoscale resolution. This anti-Brownian electrophoretic trap (ABEL trap) works by monitoring the Brownian motion of the particle (via fluorescence microscopy), and then applying a feedback voltage to the solution so that the electrophoretic drift exactly cancels the Brownian motion. The ABEL trap works on any object that can be imaged optically and that acquires a charge in water. The ABEL trap is noninvasive, is gentle enough to handle biological molecules, and can trap objects far smaller than can be trapped with laser tweezers. Our proof-of-principle device can trap fluorescent polystyrene nanospheres with diameters down to 20 nm. (C) 2005 American Institute of Physics.