L-Carnosine reduces telomere damage and shortening rate in cultured normal fibroblasts
L-Carnosine reduces telomere damage and shortening rate in cultured normal fibroblasts
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DOI：
10.1016/j.bbrc.2004.09.136
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发表时间：
2004-11-12
影响因子：
3.1
通讯作者：
Tan, Z
中科院分区：
文献类型：
--
作者：
Shao, L;Li, QH;Tan, Z
端粒是染色体末端的重复DNA序列，随着细胞分裂而逐渐缩短，限制了正常人体细胞的复制潜能。L-肌肽是一种天然存在的二肽，据报道，它可以延缓人二倍体成纤维细胞的复制性衰老，延长其寿命。本文研究了肌肽对培养的人胚肺成纤维细胞端粒DNA的影响。在20 mM肌肽中连续生长的细胞表现出较慢的端粒缩短速率和延长的寿命。当保持在一个长期的非增殖状态，他们积累了少得多的损害，在端粒DNA培养时，肌肽的存在。我们认为，端粒缩短率的降低和端粒DNA的损伤对肌肽的延长寿命作用做出了重要贡献。(C)2004年爱思唯尔公司All rights reserved.
Telomere is the repetitive DNA sequence at the end of chromosomes, which shortens progressively with cell division and limits the replicative potential of normal human somatic Cells. L-Carnosine, a naturally occurring dipeptide, has been reported to delay the replicative senescence, and extend the lifespan of cultured human diploid fibroblasts. In this work, we studied the effect of carnosine on the telomeric DNA of cultured human fetal lung fibroblast cells. Cells continuously grown in 20 mM carnosine exhibited a slower telomere shortening rate and extended lifespan in population doublings. When kept in a long-term nonproliferating state, they accumulated much less damages in the telomeric DNA when cultured in the presence of carnosine. We suggest that the reduction in telomere shortening rate and damages in telomeric DNA made an important contribution to the life-extension effect of carnosine. (C) 2004 Elsevier Inc. All rights reserved.