Imaging cone photoreceptors in three dimensions and in time using ultrahigh resolution optical coherence tomography with adaptive optics.
Imaging cone photoreceptors in three dimensions and in time using ultrahigh resolution optical coherence tomography with adaptive optics.
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DOI：
10.1364/boe.2.000748
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发表时间：
2011-03-01
影响因子：
3.4
通讯作者：
Miller DT
中科院分区：
文献类型：
--
作者：
Kocaoglu OP;Lee S;Jonnal RS;Wang Q;Herde AE;Derby JC;Gao W;Miller DT
最近已经使用自适应光学相干断层成像术在所有三个空间维度中以微米级分辨率对活体人眼中的锥状光感受器进行成像。虽然这些进展允许对活体人类视锥细胞的三维结构进行非侵入性研究，但随着时间的推移监测相同细胞的困难仍然阻碍了对其功能和生理学的研究。本研究的目的是证明使用超高分辨率自适应光学光学相干断层扫描仪进行锥体监测的可行性。这是一个高速CMOS相机（125 KHz）和一种新的基于特征的图像配准/去扭曲算法，用于减少眼睛运动对体积图像的有害影响的结合的关键。在视网膜偏心率为0.5°至6°的情况下，对三名健康受试者拍摄了体积电影。图像配准/去扭曲将电影中的运动伪影从15 μm均方根减少到1.3 μm均方根，后者足以识别和跟踪锥体。锥行间距和外节长度与文献报道一致。视锥长度分析表明，UHR-AO-OCT对于测量相同0.5°视网膜斑中的视锥之间的真实的长度差异具有足够的灵敏度，并且需要不超过5次OS长度测量以达到95%置信度。我们知道没有其他成像方式可以监测中心凹或旁视锥随着时间的推移，在所有三个维度的分辨率相当。
Cone photoreceptors in the living human eye have recently been imaged with micron-scale resolution in all three spatial dimensions using adaptive optics optical coherence tomography. While these advances have allowed non-invasive study of the three-dimensional structure of living human cones, studies of their function and physiology are still hampered by the difficulties to monitor the same cells over time. The purpose of this study is to demonstrate the feasibility of cone monitoring using ultrahigh-resolution adaptive optics optical coherence tomography. Critical to this is incorporation of a high speed CMOS camera (125 KHz) and a novel feature-based, image registration/dewarping algorithm for reducing the deleterious effects of eye motion on volume images. Volume movies were acquired on three healthy subjects at retinal eccentricities from 0.5° to 6°. Image registration/dewarping reduced motion artifacts in the movies from 15 μm to 1.3 μm root mean square, the latter sufficient for identifying and tracking cones. Cone row-to-row spacing and outer segment lengths were consistent with that reported in the literature. Cone length analysis demonstrates that UHR-AO-OCT is sufficiently sensitive to measure real length differences between cones in the same 0.5° retinal patch, and requires no more than five measurements of OS length to achieve 95% confidence. We know of no other imaging modality that can monitor foveal or parafoveal cones over time with comparable resolution in all three dimensions.