Effects of cytokines on alkaline phosphatase and osteocalcin production, calcification and calcium release by human osteoblastic cells.
Effects of cytokines on alkaline phosphatase and osteocalcin production, calcification and calcium release by human osteoblastic cells.
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DOI：
10.1093/rheumatology/33.3.224
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发表时间：
1994-03
期刊：
影响因子：
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通讯作者：
T. Kuroki;M. Shingu;Y. Koshihara;M. Nobunaga
中科院分区：
文献类型：
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作者：
T. Kuroki;M. Shingu;Y. Koshihara;M. Nobunaga
我们检测了TNF-α、IL-1 β和IL-6对从人骨膜获得的人成骨细胞培养物中碱性磷酸酶（ALP）和骨钙素（OC）产生、钙化和钙（Ca）释放的影响。将细胞与不同浓度的细胞因子一起培养3天。TNF-α和IL-1 β显著抑制人成骨细胞ALP的产生，降低细胞Ca含量，并显著增强45 Ca的释放。另一方面，IL-6显著抑制成骨细胞释放45 Ca。这些细胞因子不影响成骨细胞产生OC。所获得的结果表明，TNF-α和IL-1 β可能抑制骨形成和钙化，并且IL-6对成骨细胞的作用可能不同于TNF-α或IL-1 β。这些对成骨细胞的影响可能是RA患者发生骨丢失的机制之一。
We examined the effect of TNF-alpha, IL-1 beta and IL-6 on alkaline phosphatase (ALP) and osteocalcin (OC) production, calcification and calcium (Ca) release in human osteoblastic cell cultures obtained from human periosteum. The cells were cultured with varying concentrations of cytokines for 3 days. TNF-alpha and IL-1 beta significantly inhibited ALP production, decreased cellular Ca content, and significantly enhanced 45Ca release in human osteoblastic cells. IL-6, on the other hand, significantly suppressed 45Ca release by osteoblastic cells. These cytokines did not influence the production of OC by osteoblastic cells. The results obtained suggest that TNF-alpha and IL-1 beta may inhibit bone formation and calcification and that the effects of IL-6 on osteoblastic cells may be different from those of TNF-alpha or IL-1 beta. These effects on osteoblastic cells may be one of the mechanisms by which bone loss occurs in patients with RA.