Estrogen reduces the neurite growth of serotonergic cells expressing estrogen receptors
Estrogen reduces the neurite growth of serotonergic cells expressing estrogen receptors
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DOI：
10.1016/j.neures.2004.06.010
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发表时间：
2004-09-01
影响因子：
2.9
通讯作者：
Kawata, M
中科院分区：
文献类型：
--
作者：
Lu, HP;Nishi, M;Kawata, M
中枢神经系统的5-羟色胺能神经支配具有性别二型性。下丘脑的5-羟色胺水平受雌激素的调节。在围产期的性激素作用下，下丘脑5-羟色胺能神经支配的性二型性的形成。在本研究中，我们观察了雌激素对胚胎前14天(E14)大鼠中脑5-羟色胺能神经元轴突生长的直接影响。苯甲酸雌二醇(E_2，10(-8)M)处理7d后，5-羟色胺免疫反应阳性(IR)细胞的轴突总长度较未处理组显著缩短。逆转录-聚合酶链式反应(RT-PCR)检测E14中脑组织中雌激素受体(ER)α和ERβmRNA的表达，双荧光免疫组织化学方法检测培养的5-羟色胺-IR细胞中ERα和ERβ蛋白的表达。我们的结果提示，雌二醇对表达ERα或ERβ的5-羟色胺能细胞轴突生长的抑制作用可能与5-羟色胺能神经支配的性别二态分布的形成有关。(C)2004年爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
Serotonergic innervation of the central nervous system has a sexual dimorphism. The serotonin level in the hypothalamus was modulated by estrogen. and the formation of sexual dimorphism of serotonergic fiber innervation in the hypothalamus has been shown by the effect of sexual hormone during the critical perinatal period. In this study, we examined the direct effect of estrogen on the neurite growth of serotonergic neurons in primary culture front embryonic day 14 (E14) of rat mesencephalon. The total neurite length of serotonin-immunoreactive (IR) cells was significantly decreased by estradiol benzoate (E2, 10(-8) M) treatment for 7 days, compared with the case of no treatment. Moreover, the presence of estrogen receptor (ER) alpha and ERbeta mRNA in the E14 mesencephalon with reverse transcription-polymerase chain reaction (RT-PCR), and the ERalpha or ERbeta protein in the cultured serotonin-IR cells with double fluorescence immunohistochemistry were also demonstrated. Our results suggest that the inhibitory effects of E2 on the neurite growth of serotonergic cells expressing ERalpha or ERbeta might be involved in the formation of the sexual dimorphic distribution of serotonergic innervation. (C) 2004 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.