Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor
Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor
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DOI：
10.1590/s1516-8913201400276
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发表时间：
2015-04-01
影响因子：
1
通讯作者：
Ghiasvand, Taiebeh
中科院分区：
文献类型：
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作者：
Habibi, Peyman;Piri, Khosro;Ghiasvand, Taiebeh
利用颠茄毛状根系统提高颠茄中东莨菪碱的含量。利用发根农杆菌ATCC 15834产生毛状根。在1.5升生物反应器中进行培养，使用0.5 g fr. wt的10天大的毛状根的接种量，并评价各种参数，包括搅拌、通气、电导率和蔗糖消耗。结果表明，最高量的东莨菪碱生产（1.59毫克/克（-1）干重）发生在通气和搅拌1.25 vvm（体积每体积每分钟）和70 rpm的生物反应器，分别。电导率和蔗糖消耗的研究表明，蔗糖消耗量和矿物质消耗量最高的也是1.25 vvm和70 rpm。通过聚合酶链反应（PCR）证实转基因毛状根系。
The aim of this study was to use the, hairy root system for increasing the scopolamine content in Atropa belladonna. Agrobacterium rhizogenes ATCC15834 was utilized to produce hairy roots. The culture was carried out in a 1.5-l bioreactor using the inoculum size of 0.5 g fr. wt of 10-day-old hairy roots and various parameters, including agitation, aeration, conductivity and the consumption of sucrose were evaluated. Results revealed that the highest amount of scopolamine production (1.59 mg/g(-1) dry wt) occurred in the bioreactor with aeration and agitation 1.25 vvm (volume per volume per minute) and 70 rpm, respectively. Study of conductivity and the consumption of sucrose showed that the highest amount of sucrose consumption and the highest amount of minerals consumption also was at 1.25 vvm and 70 rpm. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR).