Delta-6-desaturase activity and arachidonic acid synthesis are increased in human breast cancer tissue
Delta-6-desaturase activity and arachidonic acid synthesis are increased in human breast cancer tissue
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DOI：
10.1111/cas.12129
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发表时间：
2013-06-01
期刊：
影响因子：
5.7
通讯作者：
Kang, Jing X.
中科院分区：
文献类型：
--
作者：
Pender-Cudlip, Marilla C.;Krag, Karen J.;Kang, Jing X.
已知ω-6（n-6）花生四烯酸（AA）及其促炎代谢物（包括前列腺素E2（PGE 2））促进肿瘤发生。δ-6去饱和酶（D 6D）是将n-6亚油酸（LA）转化为AA的限速酶。我们的目的是确定AA合成，特别是D 6D活性和PGE 2水平是否在癌性乳腺组织中增加，以及这些变量在雌激素受体阳性（ER+）和阴性（ER）乳腺癌之间是否存在差异。对从69名患有乳腺癌的妇女收集的手术乳腺组织样品进行气相色谱分析。54例ER+乳腺癌，15例ER乳腺癌。采用液相色谱-质谱法测定前列腺素E2水平。脂质分析显示癌组织中LA代谢产物（C18：3 n-6、C20：3 n-6和AA）的水平高于癌旁组织（P
Omega-6 (n-6) arachidonic acid (AA) and its pro-inflammatory metabolites, including prostaglandin E2 (PGE2), are known to promote tumorigenesis. Delta-6 desaturase (D6D) is the rate-limiting enzyme for converting n-6 linoleic acid (LA) to AA. Our objective was to determine if AA synthesis, specifically D6D activity, and PGE2 levels are increased in cancerous breast tissue, and whether these variables differ between estrogen receptor positive (ER+) and negative (ER) breast cancers. Gas chromatography was performed on surgical breast tissue samples collected from 69 women with breast cancer. Fifty-four had ER+ breast cancer, and 15 had ER breast cancer. Liquid chromatography-mass spectrometry was used to determine PGE2 levels. Lipid analysis revealed higher levels of LA metabolites (C18:3 n-6, C20:3 n-6, and AA) in cancerous tissue than in adjacent noncancerous tissue (P