MicroRNA-21 is overexpressed in pancreatic cancer and a potential predictor of survival.
MicroRNA-21 is overexpressed in pancreatic cancer and a potential predictor of survival.
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MicroRNA-21 在胰腺癌中过度表达，是生存的潜在预测因子。
DOI：
10.1007/s11605-008-0584-x
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发表时间：
2008-12
影响因子：
3.2
通讯作者：
Bloomston, Mark
中科院分区：
文献类型：
--
作者：
Dillhoff, Mary;Liu, James;Frankel, Wendy;Croce, Carlo;Bloomston, Mark
MicroRNA是一类小分子（18-22个核苷酸）非编码RNA，参与多种靶基因的转录后修饰。其中之一，microRNA-21（miR-21），已被证明在多种血液和实体器官恶性肿瘤中发挥作用。我们试图确定miR-21在胰腺癌中的表达模式及其对临床病理特征的影响。显微解剖80个切除的胰腺癌标本，并一式两份创建组织微阵列（TMA）。还为良性胰腺（N=12）和慢性胰腺炎（N=45）创建了TMA。利用miR-21的锁核酸探针进行原位杂交（ISH）。RNA U6和乱序RNA分别作为阳性和阴性对照。ISH评分为0（不存在）、1+（微弱/局灶性表达）或2+（强表达）。构建Kaplan-Meier生存曲线，并通过对数秩分析进行比较。miR-21表达在63例胰腺癌（79%）中得到证实（49例中1+，14例中2+），而良性胰腺癌和慢性胰腺炎分别为12例（8%，p<0.0001）和12/45例（27%，p<0.0001）。良性组织中没有一个表现出强的miR-21表达。尽管miR-21表达与肿瘤大小、分化程度、淋巴结状态或T分期无关，但在淋巴结阴性疾病患者中，与miR-21表达缺失或微弱/局灶性表达相比，miR-21强表达预测预后较差（中位数27.7个月vs. 15.2个月，p=0.037）。淋巴结状态也可预测生存率（p=0.029）。MicroRNA-21在胰腺癌中显著过表达，如通过原位杂交检测到的。它的强表达预示着淋巴结阴性疾病患者的生存期有限，可能是预后的重要生物标志物。
MicroRNAs are small (18–22 nucleotides) noncoding RNAs involved in posttranscriptional modification of many target genes. One of these, microRNA-21 (miR-21), has been shown to play a role in multiple hematologic and solid organ malignancies. We sought to determine the expression pattern of miR-21 in pancreatic cancers and its impact on clinicopathologic characteristics. Eighty resected pancreatic cancer specimens were microdissected and tissue microarrays (TMA) created in duplicate. TMAs were also created for benign pancreas (N=12) and chronic pancreatitis (N=45). In situ hybridization (ISH) was undertaken utilizing locked nucleic acid probes for miR-21. RNA U6 and scrambled RNA served as positive and negative control, respectively. ISH was scored as 0 (absent), 1+ (faint/focal expression), or 2+ (strong expression). Kaplan– Meier survival curves were constructed and compared by log-rank analysis. MiR-21 expression was demonstrated in 63 (79%) pancreatic cancers (1+ in 49, 2+ in 14) compared to one of 12 (8%, p<0.0001) benign pancreas and 12/45 (27%, p<0.0001) chronic pancreatitis. None of the benign tissues demonstrated strong miR-21 expression. Although miR-21 expression did not correlate with tumor size, differentiation, nodal status, or T stage, strong miR-21 expression was predictive of poorer outcome compared to absent or faint/focal miR-21 expression in patients with node-negative disease (median 27.7 months vs. 15.2, p=0.037). Nodal status was also predictive of survival (p=0.029). MicroRNA-21 is significantly overexpressed in pancreatic cancers as detected by in situ hybridization. Its strong expression predicts limited survival in patients with node-negative disease and may be an important biologic marker for outcome.
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影响因子：
120.7
作者：
Schetter, Aaron J.;Leung, Suet Yi;Harris, Curtis C.
通讯作者：
Harris, Curtis C.
影响因子：
6.4
作者：
Lee, Eun Jon;Gusev, Yuriy;Schmittgen, Thomas D.
通讯作者：
Schmittgen, Thomas D.
影响因子：
4.5
作者：
Lee, Eun Joo;Baek, Myungwon;Schmittgen, Thomas D.
通讯作者：
Schmittgen, Thomas D.
影响因子：
9
作者：
Yeo, CJ;Abrams, RA;Cameron, JL
通讯作者：
Cameron, JL
DOI：
10.1016/j.jtcvs.2007.08.055
发表时间：
2008-02-01
影响因子：
6
作者：
Feber, Andrew;Xi, Liqiang;Litle, Virginia R.
通讯作者：
Litle, Virginia R.