Two RNA species co‐purify with RNase P from the fission yeast Schizosaccharomyces pombe.
Two RNA species co‐purify with RNase P from the fission yeast Schizosaccharomyces pombe.
复制标题
两种 RNA 物种与来自裂殖酵母裂殖酵母的 RNase P 共纯化。
作者：
G. Krupp;B. Cherayil;D. Frendewey;S. Nishikawa;D. Söll
裂糖菌RNase P活性与两种RNA共纯化。通过微球菌核酸酶失活实验判断，这些rna与酶活性相关。这两个RNA， K1‐和K2‐RNA，分别有285和270个核苷酸长。这两种rna都是从一个基因转录而来，存在于单倍体基因组的一个拷贝中。已经确定了K RNA的一级和二级结构，并与来自大肠杆菌的M1 RNA进行了比较。观察到非常有限的序列同源性，这与在酵母基因组DNA的Southern分析中检测不到与M1 RNA交叉杂交的发现一致。然而，K RNA和M1 RNA的二级结构显示相同的基本组织和一个保守的局部基序，序列GUG‐‐AGGPu在一个暴露的发夹环中。
RNase P activity from Schizosaccharomyces pombe co‐purifies with two RNA species. These RNAs are associated with enzyme activity as judged by titrated micrococcal nuclease inactivation experiments. The two RNAs, K1‐ and K2‐RNA, are 285 and 270 nucleotides long, respectively. Both RNAs are transcribed from one gene, present in a single copy in the haploid genome. The primary and a secondary structure of K RNAs have been determined and compared with M1 RNA, their counterpart from Escherichia coli. Very limited sequence homology was observed, and this agrees with the finding that no cross‐hybridization with M1 RNA can be detected in a Southern analysis with yeast genomic DNA. However, the secondary structures of K RNA and M1 RNA show the same basic organization and one conserved local motif, the sequence GUG‐‐AGGPu in an exposed hairpin loop.