Nickel mine spoils revegetation attempts:: effect of pioneer plants on two functional bacterial communities involved in the N-cycle
Nickel mine spoils revegetation attempts:: effect of pioneer plants on two functional bacterial communities involved in the N-cycle
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DOI：
10.1111/j.1462-2920.2005.00705.x
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发表时间：
2005-04-01
影响因子：
5.1
通讯作者：
Navarro, E
中科院分区：
文献类型：
--
作者：
Héry, M;Philippot, L;Navarro, E
新喀里多尼亚的镍矿废料代表了一种极端的环境，富含镍，但严重缺乏碳和氮等元素。为了恢复这些网站，重新植被的尝试进行与当地植物物种建立二氮固定共生（Gymnostoma webbianum和Serianthes calycina）。由于这种生物固定过程在这种极端环境中提供了可用氮的主要来源，因此可以预期氮循环将受到刺激。为了研究矿山废弃物的植被恢复效果，研究了两种先锋植物对两种参与氮循环的功能细菌群落结构和活性的影响。nifH和narG基因分别作为二氮固定菌和异化硝酸盐还原菌的分子标记。为了评估植物对两个群落的影响，为每个靶基因构建了9个克隆文库。分别含有602和513 nifH和narG克隆的文库通过限制性片段长度多态性（RFLP）分析进行筛选。分别从nifH和narG克隆文库中对来自至少所有含有一个以上克隆的RFLP家族的141个和78个代表性克隆进行测序。这两种先锋植物都改变了两个功能群落的多样性和活性。然而，不同的影响，观察到的植物物种和社区的考虑。Serianthes calycina强烈地选择了固氮菌的一种类型，并恢复了两个社区的潜在活动。与此相反，G. webbianum没有选择任何特定的类型，只恢复了一个修复活动。
Nickel mine spoils in New Caledonia represent an extreme environment, rich in nickel and strongly deficient in elementary elements such as carbon and nitrogen. To rehabilitate these sites, revegetation attempts are performed with endemic plant species establishing dinitrogen-fixation symbiosis (Gymnostoma webbianum and Serianthes calycina). As this biological fixation process provides the major source of available nitrogen in this extreme environment, it could be expected that nitrogen cycling would be stimulated. To study the revegetation effect on mine spoils, the effect of the two pioneer plants on the structure and activity of two functional bacterial communities involved in the N-cycle was investigated. nifH and narG genes were used as molecular markers for dinitrogen-fixers and dissimilatory nitrate reducers respectively. In order to assess the influence of the plants on both communities, nine clone libraries were constructed for each targeted gene. Libraries containing 602 and 513 nifH and narG clones, respectively, were screened by restriction fragment length polymorphism (RFLP) analysis. One hundred and forty-one and 78 representative clones from at least all RFLP families containing more than one clone were sequenced from nifH and narG clone libraries respectively. Both pioneer plants modified the diversity and activity of the two functional communities. However, distinct effects were observed depending on the plant species and the community considered. Serianthes calycina strongly selected a diazotroph phylotype and restored the potential activity of both communities. In contrast, G. webbianum selected no particular phylotype and only restored a fixing activity.