Novel envelope determinants for CCR3 use by human immunodeficiency virus
Novel envelope determinants for CCR3 use by human immunodeficiency virus
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DOI：
10.1128/jvi.01030-06
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发表时间：
2006-11-01
影响因子：
5.4
通讯作者：
McKnight, Aine
中科院分区：
文献类型：
--
作者：
Aasa-Chapman, Marlen M. I.;Seymour, Craig R.;McKnight, Aine
人类免疫缺陷病毒1型通常可以使用CCR 3和CCR 5进入细胞。我们发现，信封与新的表型出现在“辅助受体。开关”：一个丧失了使用CCR 3的能力（仅R5表型），另一个获得了除了CCR 5和CCR 3之外的CXCR 4的使用（R3/R5/X4使用表型）。CCR 3的包膜决定簇定位于三个氨基酸。一个是保守区3中的N356，是一个潜在的糖基化位点，以前没有与辅助受体的使用相关。另外两个，保守区4中的R440和N448，与已经鉴定为对CCR 5结合重要的残基接近但不同。
Human immunodeficiency virus type 1 can generally use CCR3 and CCR5 for cell entry. We show that envelopes with novel phenotypes arise during "coreceptor. switch": one loses the ability to use CCR3 (R5-only phenotype), and another gains use of CXCR4 in addition to CCR5 and CCR3 (R3/R5/X4-using phenotype). The envelope determinants for CCR3 use mapped to three amino acids. One, N356 in conserved region 3, is a potential glycosylation site and has not previously been associated with coreceptor use. The other two, R440 and N448 in conserved region 4, are proximal to but distinct from residues already identified as being important for CCR5 binding.