Studies on the rate and site-specificity of P element transposition.
Studies on the rate and site-specificity of P element transposition.
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P元件转座率和位点特异性的研究。
DOI：
10.1093/genetics/127.3.515
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发表时间：
1991
期刊：
影响因子：
3.3
通讯作者：
Spradling,AC
中科院分区：
文献类型：
--
作者：
Berg,CA;Spradling,AC
一个单一的遗传标记的P元件可以有效地动员插入诱变果蝇基因组。我们已经研究了起始元件的结构及其沿X染色体沿着的位置如何影响突变恢复的速率和位置。两个P[rosy+]元素的结构强烈影响了自主“Jumpstarter-1”元素的动员。它们的平均转座率相差超过12倍，而它们的初始染色体位置的影响较小。将通过从位置1F移动P[rosy+]元件诱导的致死和不育突变与先前使用位置9 C处的P[neoR]元件鉴定的那些进行比较。除了一个可能的例外，一个元件的插入热点通常也是另一个转座子的靶标。这些实验表明，P元件的基因组位置通常不会影响其在非同源染色体上的靶位点。在这些实验的过程中，Y-连锁插入表达玫瑰色+被回收，这表明标记的P元素有时可以插入异染色质位点并发挥作用。
A single genetically marked P element can be efficiently mobilized to insertionally mutagenize the Drosophila genome. We have investigated how the structure of the starting element and its location along the X chromosome influenced the rate and location of mutations recovered. The structure of two P[rosy+] elements strongly affected mobilization by the autonomous "Jumpstarter-1" element. Their average transposition rates differed more than 12-fold, while their initial chromosomal location had a smaller effect. The lethal and sterile mutations induced by mobilizing a P[rosy+] element from position 1F were compared with those identified previously using a P[neoR] element at position 9C. With one possible exception, insertion hotspots for one element were frequently also targets of the other transposon. These experiments suggested that the genomic location of a P element does not usually influence its target sites on nonhomologous chromosomes. During the course of these experiments, Y-linked insertions expressing rosy+ were recovered, suggesting that marked P elements can sometimes insert and function at heterochromatic sites.