Variable Nanoparticle-Cell Adhesion Strength Regulates Cellular Uptake
Variable Nanoparticle-Cell Adhesion Strength Regulates Cellular Uptake
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DOI：
10.1103/physrevlett.105.138101
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发表时间：
2010-09-21
影响因子：
8.6
通讯作者：
Zhang, Sulin
中科院分区：
文献类型：
--
作者：
Yuan, Hongyan;Li, Ju;Zhang, Sulin
在受体介导的内吞作用中，细胞对粘附机制进行生化控制以吞噬外来颗粒，其特征在于可变的粘附强度。在这里，我们提出了一个热力学模型，我们阐明，可变的粘附强度严格控制细胞的摄取，产生的配体密度和粒度的空间中的摄取相图。我们从图中识别出具有显著高摄取的内吞相，所述内吞相由分别由粘附强度的熵和熵极限设定的相边界的下限和上限所包围。该相图可为合理设计基于纳米颗粒的治疗和诊断剂提供有用的指导。
In receptor-mediated endocytosis, cells exercise biochemical control over the mechanics of adhesion to engulf foreign particles, featuring a variable adhesion strength. Here we present a thermodynamic model with which we elucidate that the variable adhesion strength critically governs the cellular uptake, yielding an uptake phase diagram in the space of ligand density and particle size. We identify from the diagram an endocytosed phase with markedly high uptake, encompassed by a lower and an upper phase boundary that are set, respectively, by the enthalpic and entropic limits of the adhesion strength. The phase diagram may provide useful guidance to the rational design of nanoparticle-based therapeutic and diagnostic agents.