Comparison of the Expression Levels of Napsin A, Thyroid Transcription Factor-1, and p63 in Nonsmall Cell Lung Cancer Using Cytocentrifuged Bronchial Brushings
Comparison of the Expression Levels of Napsin A, Thyroid Transcription Factor-1, and p63 in Nonsmall Cell Lung Cancer Using Cytocentrifuged Bronchial Brushings
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DOI：
10.1002/cncy.20162
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发表时间：
2011-10-25
影响因子：
3.4
通讯作者：
Kage, Masayoshi
中科院分区：
文献类型：
--
作者：
Aikawa, Emiko;Kawahara, Akihiko;Kage, Masayoshi
背景：非小细胞肺癌（NSCLC）的适当治疗取决于组织学类型。本研究的目的是评估Napsin A，甲状腺转录因子-1（TTF-1），和p63的表达水平的免疫染色使用CytoRich Red保存细胞离心支气管刷。方法：在12例同时获得支气管刷检细胞学和支气管活检样本的患者中，以及在64例切除的NSCLC组织样本和8例正常或良性支气管刷检细胞学样本中，使用比例和强度评分半定量评估Napsin A、TTF-1和p63的表达水平。研究结果：TTF-1和p63的核表达允许癌细胞的良好可视化，而Napsin A在癌细胞中的胞质表达通常更难以评估，因为中型和大型巨噬细胞也表达Napsin A。在细胞学和组织学样本中，Napsin A和TTF-1是可靠的腺癌标志物，p63是可靠的鳞状细胞癌标志物。在支气管刷检细胞学和支气管活检标本中，Napsin A、TTF-1和p63的平均评分高度相关，而在切除的NSCLC组织标本中，TTF-1表达显著低于Napsin A表达（P < .001）。结论：CytoRich Red固定的支气管刷免疫染色有助于确定NSCLC的组织学类型。提示Napsin A、TTF-1和p63的组合可有效识别NSCLC的组织学类型，应根据形态学选择Napsin A和p63或TTF-1和p63的组合。癌症（癌症细胞病理学）2011;119：335-45。(C)2011年美国癌症协会
BACKGROUND: Appropriate treatment of nonsmall cell lung cancer (NSCLC) depended on histologic type. The aim of this study was to evaluate the expression levels of Napsin A, thyroid transcription factor-1 (TTF-1), and p63 by immunostaining using CytoRich Red preserved cytocentrifuged bronchial brushings. METHODS: The expression levels of Napsin A, TTF-1, and p63 were semiquantitatively evaluated using proportion and intensity scores in 12 patients from whom both bronchial brushing cytology and bronchial biopsy samples had been obtained, as well as in a selection of 64 resected NSCLC tissue samples, and 8 normal or benign bronchial brushing cytology samples. RESULTS: The nuclear expressions of TTF-1 and p63 allowed for good visualization of cancer cells, whereas the cytoplasmic expression of Napsin A in cancer cells was often more difficult to evaluate because medium-sized and large macrophages also expressed Napsin A. Napsin A and TTF-1 were reliable adenocarcinoma markers, and p63 was a reliable squamous cell carcinoma marker in cytology, and histology samples. The average scores of Napsin A, TTF-1, and p63 were highly correlated in bronchial brushing cytology and bronchial biopsy samples, whereas TTF-1 expression was significantly lower than Napsin A expression in resected NSCLC tissue samples (P < .001). CONCLUSIONS: Immunostaining of bronchial brushings fixed with CytoRich Red was useful in determining histologic types in NSCLC. It was suggested that the panels of Napsin A, TTF-1, and p63 were effective in identifying histologic type in NSCLC, and that a combination of either Napsin A and p63 or TTF-1 and p63 should be chosen, depending on morphology. Cancer (Cancer Cytopathol) 2011;119:335-45. (C) 2011 American Cancer Society.