Easy, Scalable, Robust, Micropatterned Silk Fibroin Cell Substrates
Easy, Scalable, Robust, Micropatterned Silk Fibroin Cell Substrates
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DOI：
10.1002/admi.201801822
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发表时间：
2019-04-01
影响因子：
5.4
通讯作者：
Yadavalli, Varnsi K.
中科院分区：
文献类型：
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作者：
Xu, Meng;Pradhan, Sayantan;Yadavalli, Varnsi K.
聚合物薄膜正在被用于生物医学用途，如药物输送、生物过滤、生物传感器和组织再生。特别感兴趣的是机械柔性片的形成，它可以形成具有可控制厚度的密封伤口，或作为仿生细胞结构。具有精确微和纳米图案的柔性基质可以在生物界面上通过保形接触作为细胞生长的支持。迄今为止，形成独立薄片的方法在呈现图案结构和微/纳米纹理表面的能力方面受到限制。其他材料缺乏可降解性，阻碍了它们作为细胞支架的应用。提出了一种利用丝素蛋白制备生物相容性和可生物降解薄膜的方法。这项工作提出了柔性、微图案和可生物降解的二维纤维蛋白片的制备和表征，用于细胞粘附和增殖。一种使用光刻技术的简单和可扩展的技术被证明可以在大范围内制造具有可调和精确微图案的光学透明，坚固和灵活的丝素基板。通过控制表面结构，可以观察到对细胞粘附和扩散的控制。此外，基础材料可以通过蛋白质水解完全降解。通过机械控制和引导贴壁细胞，可以探索细胞与微尺度几何地形的相互作用。
Thin polymeric films are being explored for biomedical uses such as drug delivery, biofiltration, biosensors, and tissue regeneration. Of specific interest is the formation of mechanically flexible sheets, which can be formed with controllable thickness for sealing wounds, or as biomimetic cellular constructs. Flexible substrates with precise micro- and nanopatterns can function as supports for cell growth with conformal contact at the biointerface. To date, approaches to form free-standing, thin sheets are limited in the ability to present patterned architectures and micro/nanotextured surfaces. Other materials have a lack of degradability, precluding their application as cellular scaffolds. An approach is suggested using biocompatible and biodegradable films fabricated from silk fibroin. This work presents the fabrication and characterization of flexible, micropatterned, and biodegradable 2D fibroin sheets for cell adhesion and proliferation. A facile and scalable technique using photolithography is shown to fabricate optically transparent, strong, and flexible fibroin substrates with tunable and precise micropatterns over large areas. By controlling the surface architectures, the control of cell adhesion and spreading can be observed. Additionally, the base material is fully degradable via proteolysis. Through mechanical control and directing the adherent cells, it is possible to explore interactions of cells and the microscale geometric topography.