Involvement of protein kinase C signalling and mitogen-activated protein kinase in the amebocyte-producing organ of Biomphalaria glabrata (Mollusca)
Involvement of protein kinase C signalling and mitogen-activated protein kinase in the amebocyte-producing organ of Biomphalaria glabrata (Mollusca)
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DOI：
10.1016/j.dci.2009.01.001
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Sullivan, John T.
中科院分区：
文献类型：
--
作者：
Salamat, Zahra;Sullivan, John T.
在生物体或细胞水平上调节软体动物血细胞产生的机制尚不清楚。在本研究中，24小时盐水培养的阿米巴细胞产生的器官（APO）的寄生虫传播蜗牛Biomphalaria光滑被用来测试潜在的参与蛋白激酶C（PKC）信号在造血。暴露于佛波醇肉豆蔻酸酯（PMA），PKC的激活剂，导致在APOs中的分裂造血细胞的数量增加，从抗线粒体萨尔瓦多蜗牛。PMA诱导的细胞分裂被阻断治疗与U 0126，抑制剂的促分裂原活化蛋白激酶激酶，MEK 1/2。这些结果表明，PKC诱导的有丝分裂原活化蛋白激酶，ERK 1/2，参与细胞分裂的载脂蛋白O。(C)2009爱思唯尔有限公司保留所有权利。
Mechanisms that regulate hemocyte production in molluscs, at either the organismal or cellular levels, are not well understood. In the present study, 24-h saline cultures of the amebocyte-producing organ (APO) of the schistosome-transmitting snail Biomphalaria glabrata were used to test for the potential involvement of protein kinase C (PKC) signalling in hematopoiesis. Exposure to phorbol myristate acetate (PMA), an activator of PKC, resulted in an increase in the number of dividing hematopoietic cells in APOs from schistosome-resistant Salvador snails. PMA-induced cell division was blocked by treatment with U0126, an inhibitor of the mitogen-activated protein kinase kinase, MEK1/2. These results suggest that PKC-induced activation of the mitogen-activated protein kinase, ERK1/2, is involved in cell division in the APO. (C) 2009 Elsevier Ltd. All rights reserved.