Transcriptome profile analysis reveals the ontogenesis of rooted chichi in Ginkgo biloba L.
Transcriptome profile analysis reveals the ontogenesis of rooted chichi in Ginkgo biloba L.
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转录组谱分析揭示了银杏树根chichi的个体发生。
DOI：
10.1016/j.gene.2018.05.066
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发表时间：
2018-08-30
期刊：
影响因子：
3.5
通讯作者：
Xing, Shiyan
中科院分区：
文献类型：
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作者：
Liu, Xiaojing;Sun, Limin;Xing, Shiyan
银杏（Ginkgo biloba L.）奇奇是一种独特的器官。目的：探讨G.为了研究银杏叶片的基因表达谱，我们使用RNA-seq分析了两个发育阶段（ch 1和ch 2）和附近组织（ck）的生根奇奇的转录组谱，每个样品有三个生物学重复。在9个cDNA文库中共产生了57.74Gb的干净碱基。这些碱基从头组装成68，277个unigenes，平均长度为844 bp，51.47%的unigenes在至少一个公共数据库中具有匹配。在ch 1与ck和ch 2与ck中的差异表达基因（DEG）分别为2748和8594。参与生长素信号通路、生长素极性运输、贮藏相关蛋白和细胞周期通路的DEG可能在奇奇的个体发育中发挥作用。实时定量PCR结果与转录组数据密切相关。本研究中获得的转录组资源提供了G.为探讨银杏个体发育的分子机制提供了重要的资源。biloba chichi。
The Ginkgo biloba L. chichi is a unique organ. To explore the molecular mechanisms underlying the ontogenesis of G. biloba chichi, we used RNA-seq to analyse the transcriptome profile of rooted chichi at two developmental stages (ch1 and ch2) and nearby tissues (ck), and each sample had three biological replicates. A total of 57.74 Gb of clean bases were generated in nine cDNA libraries. These bases were de novo assembled into 68,277 unigenes with average length of 844 bp, and 51.47% of the unigenes had a match in at least one public database. The differentially expressed genes (DEGs) in chl vs. ck and ch2 vs. ck were 2748 and 8594, respectively. The DEGs involved in the auxin signal pathway, auxin polar transport, storage-related proteins, and the cell cycle pathway might play roles in the ontogenesis of chichi. The quantitative real-time PCR results were closely correlated with transcriptome data. The transcriptome resources generated in the current study provide gene expression profiles and differential expression profiles of G. biloba chichi and offer an essential resource to probe the molecular mechanisms underlying the ontogenesis of G. biloba chichi.