The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants.
The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants.
复制标题
稻瘟病菌的 Nep1 样蛋白家族对于水稻植物的感染是可有可无的
DOI：
10.1038/s41598-017-04430-0
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发表时间：
2017-06-29
影响因子：
4.6
通讯作者：
Fan J
中科院分区：
文献类型：
--
作者：
Fang YL;Peng YL;Fan J
坏死和乙烯诱导蛋白1（Nep1）样蛋白（NLP）是一类广泛分布于植物相关微生物中的微生物相关分子模式。尽管在双子叶植物中具有细胞毒性活性，但大多数NLP在植物病原体毒力中的作用在很大程度上仍然未知。结果表明，稻瘟病菌MoNLP家族的氨基酸序列差异很小，三种MoNLP的瞬时表达均诱导了本氏烟草细胞死亡和活性氧的产生，并且在感病水稻植株的侵染过程中诱导了MoNLP的表达。为了进一步研究MoNLP家族的生物学作用，开发了一种无标记的基因替换载体，并用于敲除稻瘟菌的整个家族。结果表明，野生型和四重ΔMoNLP突变株引起的疾病水平没有显著差异。在营养不良和非生物胁迫等各种不利培养条件下，两株菌的产孢和径向生长情况相似。这些观察结果表明，MoNLP家族对测试的不利培养条件的真菌耐受性，更重要的是，对感病水稻植株的稻瘟病菌的毒力。
The necrosis- and ethylene-inducing protein 1 (Nep1)-like proteins (NLPs) are a class of microbe-associated molecular patterns widely distributed across diverse groups of plant-associated microorganisms. In spite of the cytotoxic activity in dicot plants, the role of most NLPs in the virulence of plant pathogens is still largely unknown. We showed that the MoNLP family of rice blast fungus varied very little in amino acid sequence, transient expression of three MoNLPs induced cell death and the production of reactive oxygen species in Nicotiana benthamiana, and the expression of MoNLPs was induced during infection of susceptible rice plants. To further investigate the biological role of the MoNLP family, a marker-free gene replacement vector was developed and used to knock out the whole family in Magnaporthe oryzae. Results showed no significant difference in disease levels caused by wild type and the quadruple ΔMoNLP mutant strains. Likewise, the sporulation and radial growth of the two strains were similar under various unfavorable cultural conditions including malnutrition and abiotic stresses. These observations demonstrated that the MoNLP family is dispensable for the fungal tolerance to the tested adverse cultural conditions, and more importantly, for the virulence of blast fungus on susceptible rice plants.