Value of Osteoblast-Derived Exosomes in Bone Diseases
Value of Osteoblast-Derived Exosomes in Bone Diseases
复制标题
成骨细胞来源的外泌体在骨疾病中的价值
DOI：
10.1097/scs.0000000000003463
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发表时间：
2017-06-01
影响因子：
0.9
通讯作者：
Mu, Xiongzheng
中科院分区：
文献类型：
--
作者：
Ge, Min;Wu, Yingzhi;Mu, Xiongzheng
目的：揭示成骨细胞源性外泌体在骨病中的价值。方法：采用超离心方法从小鼠Mc3t3上清液中分离出微泡，并进行蛋白质组学分析。结果：共检测到1536个蛋白，发现172个蛋白与骨数据库重叠。匠心通路分析显示了“骨骼和肌肉系统发育与功能、发育障碍、遗传障碍”的网络和成骨通路。网络中的EFNB1和转化生长因子β受体3，通路中的LRP6，骨形态发生蛋白受体1型，SMURF1似乎在相关骨病的外泌体研究中有价值。结论：本研究揭示了成骨细胞外泌体的含量，并探讨了其中有价值的蛋白质，为骨疾病研究提供了新的前景。
Purpose: The authors' purpose is to reveal the value of osteoblast-derived exosomes in bone diseases.Methods: Microvesicles from supernatants of mouse Mc3t3 were isolated by ultracentrifugation and then the authors presented the protein profile by proteomics analysis.Results: The authors detected a total number of 1536 proteins by mass spectrometry and found 172 proteins overlap with bone database. The Ingenuity Pathway Analysis shows network of "Skeletal and Muscular System Development and Function, Developmental Disorder, Hereditary Disorder" and pathway about osteogenesis. EFNB1 and transforming growth factor beta receptor 3 in the network, LRP6, bone morphogenetic protein receptor type-1, and SMURF1 in the pathway seemed to be valuable in the exosome research of related bone disease.Conclusions: The authors' study unveiled the content of osteoblast-derived exosome and discussed valuable protein in it which might provide novel prospective in bone diseases research.