Expression of aquaporins, vasopressin type 2 receptor, and Na+-K+-Cl– cotransporters in the rat endolymphatic sac
Expression of aquaporins, vasopressin type 2 receptor, and Na+-K+-Cl– cotransporters in the rat endolymphatic sac
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DOI：
10.1080/00016480802441754
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发表时间：
2009-01
影响因子：
1.4
通讯作者：
M. Nishimura;A. Kakigi;T. Takeda;S. Takeda;K. Doi
中科院分区：
文献类型：
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作者：
M. Nishimura;A. Kakigi;T. Takeda;S. Takeda;K. Doi
结论。由于水通道蛋白（AQP） 1 - 4、AQP6-9、抗利尿素2型受体（V2-R）、Na+- k +- cl -共转运蛋白（NKCC） 1、NKCC2等多种水通道和水泵在大鼠内淋巴囊中表达，因此在内淋巴液稳态的酸基代谢和水分平衡的生理功能中发挥着重要作用。目的：探讨AQP1-9、V2-R、NKCC1、NKCC2在大鼠内淋巴囊中的表达及免疫定位。材料和方法。采用Wistar大鼠。采用逆转录聚合酶链反应（RT-PCR）方法检测大鼠内淋巴囊AQP1-9、V2-R、NKCC1和NKCC2 mRNA的表达，并采用免疫组织化学方法（包括免疫荧光显微镜）详细检测AQP1-9、V2-R、NKCC1和NKCC2的免疫定位。结果。AQP1-4、AQP6-9、V2-R、NKCC1和NKCC2的mrna和蛋白均有表达，AQP5在大鼠内淋巴囊中未表达。
Conclusion. Since many water channels and pumps, such as aquaporin (AQP) 1–4, AQP6–9, vasopressin type 2 receptor (V2-R), Na+-K+-Cl– cotransporter (NKCC) 1, and NKCC2 were expressed in the rat endolymphatic sac, it should play an important role in the physiological function of acid-based metabolism and water balance in endolymphatic fluid homeostasis. Objective: To evaluate the expression and immunolocalization of AQP1–9, V2-R, NKCC1, and NKCC2 in the rat endolymphatic sac. Materials and methods. Wistar rats were used. Expression of AQP1–9, V2-R, NKCC1, and NKCC2 mRNA in the rat endolymphatic sac was investigated using the reverse transcription-polymerase chain reaction (RT-PCR) method, and detailed immunolocalization of AQP1–9, V2-R, NKCC1, and NKCC2 was investigated using immunohistochemical methods, including immunofluorescence microscopy. Results. mRNAs and proteins of AQP1–4, AQP6–9, V2-R, NKCC1, and NKCC2 were expressed, but AQP5 was not expressed in the rat endolymphatic sac.