Feeding habit transition induced by social learning through CaMKII signaling in Chinese perch (Siniperca chuatsi)
Feeding habit transition induced by social learning through CaMKII signaling in Chinese perch (Siniperca chuatsi)
复制标题
中华鲈鱼 (Siniperca chuatsi) 社会学习通过 CaMKII 信号诱导摄食习惯转变
DOI：
10.1016/j.aquaculture.2020.736211
复制
发表时间：
2021-02-25
期刊：
影响因子：
4.5
通讯作者：
Chen，Ke
中科院分区：
文献类型：
--
作者：
Liang，Hui;He，Shan;Chen，Ke
社会学习在获得新的觅食技能和食物偏好方面发挥着关键作用。然而，社会学习对摄食习惯影响的分子机制尚不清楚。鲈鱼是一种底栖性食鱼动物，具有独特的食性。鲈鱼鱼苗一旦开始摄食，在野外只接受活的猎物鱼，拒绝死的猎物鱼和人工饲料。本研究采用Ca 2 +/钙调蛋白依赖性蛋白激酶II（CaMKII）信号通路抑制剂KN-93，在体内外研究了社会学习对鲈鱼从活猎物向死猎物转变的影响。通过对基因表达、蛋白质磷酸化、成功率、学习次数和摄食量的分析，我们深入了解了鲈鱼社会性学习在获得新食性中的分子机制。我们的研究结果表明，在体外，KN-93处理组p-CaMK Ⅱ、磷酸化cAMP反应元件结合（p-CREB）、原癌基因c-fos（c-fos）和黑色素浓集激素（mch）的表达水平显著低于对照组，而促凋亡基因前阿黑皮素（pomc）的表达水平显著高于对照组。体内注射CaMKII抑制剂KN-93后，鲈鱼社会学习诱导的成功率和摄食量的增加均被抑制。KN-93处理后，鲈鱼的CaMK Ⅱ信号被抑制。在学习组中，-fos、mchrompomc基因表达水平升高或降低，而在KN-93注射组中，表达水平先降低后升高。这些结果表明，orexigenicmch基因的高表达和orexigenicpomc基因的低表达可能与鲈鱼对死鱼猎物的摄食学习有关。综上所述，我们的研究结果表明，社会学习可以通过CaMKII-c-fos-pomc/mch信号通路促进鲈鱼从活的猎物鱼到死的猎物鱼的食性转变。
Social learning plays pivotal roles in gaining new foraging skills and food preferences. However, the molecular mechanism underlying the effects of social learning on feeding habits is unclear. Chinese perch, as a demersal piscivore, has a unique feeding habit. Once the fry of Chinese perch start feeding, they only accept live prey fish in the wild and refuse dead prey fish or artificial diets. In the present study, we used the inhibitor of the Ca2+/calmodulin-dependent protein kinase II (CaMKII) signaling pathway KN-93in vivoandin vitro, to explore how social learning can affect the transition from live prey fish to dead prey fish in Chinese perch. Analysis of gene expression, protein phosphorylation, success rate, learning times, and food intake allowed us to gain insights into the molecular mechanism of social learning in acquiring new feeding habits in Chinese perch. Our results showed that the p-CaMKII, phospho-cAMP-response element binding (p-CREB) and proto-oncogenesc-fos(c-fos), andmelanin-concentrating hormone(mch) expression levels were significantly lower with KN-93 treatment than levels in the control condition, whereas the expression of the anorexigenic gene proopiomelanocortin (pomc) was significantly higherin vitro. The increased success rate and food intake of Chinese perch induced by social learning, were diminished after the injection of the CaMKII inhibitor KN-93in vivo. Inhibited signaling of CaMKII was observed in Chinese perch after KN-93 treatment. We also found that the expression levels of thec-fos,mchorpomcgenes were increased or decreased in the learning group and then decreased or increased in the learning group with KN-93 injection, respectively. These results indicate that the higher expression of the orexigenicmchgene and the decreased expression of the anorexigenicpomcgene might contribute to the learning of eating dead fish prey in Chinese perch. Altogether, our results showed that social learning could promote the feeding habit transition from live prey fish to dead prey fish through the CaMKII -c-fos-pomc/mchsignaling pathway in Chinese perch.