Use of monoclonal antibodies in immuno-electron microscopy for the determination of subunit stoichiometry in oligomeric enzymes. There are three alpha-subunits in the F1-ATPase of Escherichia coli.
Use of monoclonal antibodies in immuno-electron microscopy for the determination of subunit stoichiometry in oligomeric enzymes. There are three alpha-subunits in the F1-ATPase of Escherichia coli.
复制标题
在免疫电子显微镜中使用单克隆抗体来确定寡聚酶中的亚基化学计量。
DOI：
10.1016/0022-2836(84)90408-x
复制
发表时间：
1984
影响因子：
5.6
通讯作者：
H. Schairer
中科院分区：
文献类型：
--
作者：
H. Lünsdorf;K. Ehrig;P. Friedl;H. Schairer
寡聚酶的亚基化学计量可以通过免疫电子显微镜使用针对单个亚基的单克隆抗体来确定。制备了抗大肠杆菌天然F1-ATP酶α亚基的单克隆抗体。分离F1与单克隆抗体的免疫复合物。电子显微镜观察发现，每个F1-ATPase分子中含有3个免疫球蛋白。这明确地证明了E的F1-ATP酶的α 3化学计量。杆菌
The subunit stoichiometry of oligomeric enzymes can be determined by immuno-electron microscopy using monoclonal antibodies against the individual subunits. Monoclonal antibodies against native F1-ATPase ofEscherichia coliwere prepared that were specific for the α-subunit. The immune complexes of F1and monoclonal antibodies were isolated. Electron microscopy revealed the presence of three irnmunoglobulins per molecule of F1-ATPase. This unequivocally demonstrates an α3stoichiometry for the F1-ATPase ofE. coli.
大肠杆菌 H-ATP 酶复合物中亚基的化学计量。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Foster,DL;Fillingame,RH
通讯作者：
Fillingame,RH