Functional interaction of beta-catenin with the transcription factor LEF-1
Functional interaction of beta-catenin with the transcription factor LEF-1
复制标题
DOI：
10.1038/382638a0
复制
发表时间：
1996-08-15
期刊：
影响因子：
64.8
通讯作者：
Birchmeier, W
中科院分区：
文献类型：
--
作者：
Behrens, J;vonKries, JP;Birchmeier, W
脊椎动物和果蝇的细胞质蛋白β-catenin有两个功能：它们将钙粘附素细胞黏附分子连接到细胞骨架上(1-4)，并参与WNT/无翼信号通路(5-7)。在这里，我们在酵母双杂交筛选中显示，结构转录因子Lef-1(淋巴增强因子结合因子)(8-10)与β-连环蛋白相互作用。在哺乳动物细胞中，共表达的Lef-1和β-catenin形成一个位于细胞核的复合体，可以通过免疫沉淀检测到，此外，Lef-1和β-catenin与DNA形成一个三元复合体，显示出改变的DNA弯曲。将Lef-1显微注射到非洲爪哇胚胎中可以诱导轴复制，这种复制可以通过与β-连环蛋白的相互作用而增强。因此，β-连环蛋白通过与Lef-1等转录因子直接相互作用来调节基因的表达，为细胞黏附成分或ANT蛋白向细胞核传递信号提供了分子机制。
THE cytoplasmic proteins beta-catenin of vertebrates and armadillo of Drosophila have two functions: they link the cadherin cell-adhesion molecules to the cytoskeleton(1-4), and they participate in the wnt/wingless signalling pathway(5-7). Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor)(8-10) interacts with beta-catenin. In mammalian cells, coexpressed LEF-1 and beta-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation, Moreover, LEF-1 and beta-catenin form a ternary complex with DNA that displays an altered DNA bend. Microinjection of LEF-1 into Xenopus embryos induces axis duplication, which is augmented by interaction with beta-catenin. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals from cell-adhesion components or ant protein to the nucleus.