Effect and ameliorative mechanisms of polyoxometalates on the denitrification under sulfonamide antibiotics stress
Effect and ameliorative mechanisms of polyoxometalates on the denitrification under sulfonamide antibiotics stress
复制标题
多金属氧酸盐对磺胺类抗生素胁迫下反硝化作用的影响及改善机制
DOI：
10.1016/j.biortech.2020.123073
复制
发表时间：
2020-06-01
影响因子：
11.4
通讯作者：
Hou, Yanan
中科院分区：
文献类型：
--
作者：
Guo, Haixiao;Chen, Zhi;Hou, Yanan
磺胺类抗生素的环境风险近年来引起了广泛关注。本研究首次探讨了磺胺嘧啶（SDZ）对反硝化电子传递系统（ETS）的抑制作用及磷钼酸（PMo 12）的改善机理。在SDZ浓度为2 mg/L时，PMo 12使NO3--N的还原效率和速率分别从68.30%和124.22 N/g VSS/h提高到100.00%和184.59 N/g VSS/h。反硝化ETS的电子传递速率和消耗效率提高，以改善SDZ抑制，这是由于更多的分泌核黄素和细胞色素c和PMo 12介导的反硝化酶活性的增加。此外，PMo 12介导的胞外聚合物（EPS）增加，微生物生长抑制和细胞膜损伤得到改善。在SDZ胁迫下，PMo 12介导的微生物多样性增加也促进了反硝化作用的发生。本研究为改善抗生素在废水生物处理中的抑制作用提供了一种新的思路。
The environmental risks of the sulfonamide antibiotics have attracted much attention recently. In this study, the inhibition effects of sulfadiazine (SDZ) on denitrification electron transfer system (ETS) and ameliorative mechanisms of phosphomolybdic acid (PMo12) were first explored. When denitrification was under 2 mg/L SDZ stress, experiments indicated that PMo12 enhanced NO3--N reduction efficiency and rate from 68.30% to 100.00% and 124.22 to 184.59 N/g VSS/h, respectively. Electron transfer rate and consumption efficiency in denitrification ETS were enhanced to ameliorate SDZ inhibition, which was due to the more secreted riboflavin and cytochrome c and the increased denitrifying enzymes activity with PMo12 mediation. In addition, the microbial growth inhibition and cell membrane damage were ameliorated due to the more EPS surrounding microbe with PMo12 mediation. Higher diversity of denitrifying microbe with PMo12 mediation also promoted denitrification under SDZ stress. This work provided promising strategy to ameliorate antibiotics inhibition in the wastewater bio-treatment.