Saccharide detection based on the amplified fluorescence quenching of a water-soluble poly(phenylene ethynylene) by a boronic acid functionalized benzyl viologen derivative
Saccharide detection based on the amplified fluorescence quenching of a water-soluble poly(phenylene ethynylene) by a boronic acid functionalized benzyl viologen derivative
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DOI：
10.1021/la0264926
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发表时间：
2002-10-15
期刊：
影响因子：
3.9
通讯作者：
Lakowicz, JR
中科院分区：
文献类型：
--
作者：
DiCesare, N;Pinto, MR;Lakowicz, JR
阴离子共轭聚合物磺化聚（苯基乙烯基）（PPE-SO3-）被含有硼酸功能化紫苄基（p-BV2+）的阳离子猝灭剂放大猝灭，用于光学检测糖。在没有糖的情况下，强聚合物/猝灭剂相互作用导致超线性猝灭。在pH值为7.4的糖存在下，指示性紫素衍生物形成中性两性离子，降低了其与阴离子聚合物配合并抑制其释放的能力。在聚合物/猝灭剂体系中加入糖导致荧光强度大幅增加（在一个案例中高达70倍）。给出了光谱数据、淬火参数和糖滴定曲线，并对葡萄糖传感器的未来发展进行了讨论。
The amplified quenching of an anionic conjugated polymer, sulfonated poly(phenylene ethynylene) (PPE-SO3-), by a cationic quencher comprising a boronic acid functionalized benzyl viologen (p-BV2+), has been used to optically detect sugars. In the absence of sugar, a strong polymer/quencher interaction leads to superlinear quenching. In the presence of sugar at pH 7.4, the dicationic viologen derivative forms a neutral zwitterionic species, reducing its ability to complex with and quench the anionic polymer's emission. Addition of sugar to the polymer/quencher system leads a large increase in the fluorescence intensity (up to 70-fold in one case). Spectral data, quenching parameters, and sugar titration curves are presented and discussed in terms of future development of glucose sensors.