EJC-independent degradation of nonsense immunoglobulin-μ mRNA depends on 3′ UTR length
EJC-independent degradation of nonsense immunoglobulin-μ mRNA depends on 3′ UTR length
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DOI：
10.1038/nsmb1081
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发表时间：
2006-05-01
影响因子：
16.8
通讯作者：
Mühlemann, O
中科院分区：
文献类型：
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作者：
Bühler, M;Steiner, S;Mühlemann, O
与哺乳动物无义介导的mRNA衰减（NMD）的流行模型不一致的是，当最下游的内含子被删除时，倒数第二外显子中含有过早终止密码子（ptc）的免疫球蛋白-mu （Ig-mu）基因的mRNA水平仍然会被NMD降低。与酵母一样，igg -mu mrna的这种不依赖于外显子连接复合物的NMD依赖于终止密码子和聚(A)尾部之间的距离，表明PTC识别的进化保守模式。
Inconsistent with prevailing models for nonsense-mediated mRNA decay (NMD) in mammals, the mRNA levels of immunoglobulin-mu (Ig-mu) genes with premature termination codons (PTCs) in the penultimate exon are still reduced by NMD when the intron furthest downstream is deleted. As in yeast, this exon junction complex-independent NMD of Ig-mu mRNAs depends on the distance between the termination codon and the poly(A) tail and suggests an evolutionarily conserved mode of PTC recognition.