Trapping cell spheroids and organoids using digital acoustofluidics
Trapping cell spheroids and organoids using digital acoustofluidics
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DOI：
10.1088/1758-5090/ab9582
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发表时间：
2020-07-01
期刊：
影响因子：
9
通讯作者：
Guo, Feng
中科院分区：
文献类型：
--
作者：
Cai, Hongwei;Wu, Zhuhao;Guo, Feng
细胞球体和类器官的精确定位和排列对于组织工程和再生医学中复杂组织结构的重建至关重要。在这里，我们提出了一种数字声流控方法，以前所未有的灵活性操纵细胞球体和类器官。通过c形集成数字换能器（IDT）引入局部振动，我们可以生成一个捕获节点来固定直径从20 μ m到300 μ m的细胞球体。此外，我们在封闭或开放的微流控室中，在c形IDT上数字化捕获多个细胞球体。通过在3 x 3 c形IDT阵列内编程捕获节点，我们可以精确地将细胞球体定位到设计的图案中。我们还证明了我们的数字声流装置可以精确地控制球体细胞和类器官的相互作用。通过简单的制造过程和设置，我们的数字声流方法可以以非接触，无标签和高度生物相容性的方式精确地操纵和定位各种细胞球体或类器官。我们相信这项技术可以广泛应用于组织工程、再生医学和基础细胞生物学研究。
The precise positioning and arrangement of cell spheroids and organoids are critical to reconstructing complex tissue architecture for tissue engineering and regenerative medicine. Here, we present a digital acoustofluidic method to manipulate cell spheroids and organoids with unprecedented dexterity. By introducing localized vibrations via a C-shaped integrated digital transducer (IDT), we can generate a trapping node to immobilize cell spheroids withadiameters ranging from 20 mu m to 300 mu m. Moreover, we digitally trapped multiple cell spheroids atop the C-shaped IDTs within a closed or open microfluidic chamber. By programming the trapping nodes within a 3 x 3 C-shaped IDT array, we can precisely position cell spheroids into designed patterns. We also demonstrated that our digital acoustofluidic device can accurately control the interaction of spheroid cells and organoids. Along withasimple fabrication procedure and setup, our digital acoustofluidic method canprovideprecisely manipulate and position various cell spheroids or organoids in a contactless, label-free, and highly biocompatible manner. We believe this technology can be widely used for tissue engineering, regenerative medicine, and fundamental cell biology research.