Chrysochromulina andersonii sp. nov. (Prymnesiophyceae), a new flagellate haptophyte symbiotic with radiolarians

Chrysochromulina andersonii sp. nov. (Prymnesiophyceae), a new flagellate haptophyte symbiotic with radiolarians
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Chrysochromulina andersonii sp.

DOI:
10.1080/00318884.2018.1541271
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发表时间:
2019
期刊:
影响因子:
1.6
通讯作者:
Takeo Horiguchi & Osamu Takahashi
Takeo Horiguchi & Osamu Takahashi
中科院分区:
生物学3区
文献类型:
--
作者:
Tomoko Yuasa;Masanobu Kawachi;Takeo Horiguchi & Osamu Takahashi

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触生菌共生体Chrysochromulina andersonii sp。十一月使用天然和培养材料,通过光学、扫描和透射电子显微镜以及小亚基 (SSU) 和大亚基 (LSU) rDNA 序列的分子系统发育分析,对从多胱氨酸放射虫中分离得到的元宝Dictyocoryne truncatum 进行了检查。在培养中,运动细胞呈鞍形,长 3–5 µm,宽 3–4 µm。它们拥有两个等长或近等长的鞭毛、一个卷曲的触体和两个顶叶金棕色叶绿体。鳞片排列成两层,由两种类型组成:内层为扁平边缘的鳞片,直径为 0.19–0.26 µm;而直径为 0.25–0.45 µm 的具有直立边缘的鳞片形成了外层。两块鳞片的鳞面中央都有一个隐约的十字,放射状的脊线从隐约的十字向周边延伸。分子系统发育分析表明,我们的共生体的 SSU 和 LSU rDNA 序列属于 Chrysochromulina 进化枝。然而,我们的共生体在鳞片表面的图案细节上与其他金铬藻物种不同。因此,我们提出C.安德森尼氏菌十一月来自多胱氨酸放射虫的共生藻 D.元宝。
The haptophyte symbiontChrysochromulina andersonii sp. nov. isolated from the polycystine radiolarianDictyocoryne truncatumwas examined using both natural and cultured materials by light, scanning and transmission electron microscopy as well as molecular phylogenetic analyses of the small subunit (SSU) and large subunit (LSU) rDNA sequences. In culture, the motile cells were saddle-shaped, 3–5 µm long and 3–4 µm wide. They possessed two equal to subequal flagella, a coiling haptonema, and two parietal golden-brown chloroplasts. The scales were arranged in two layers composed of two types: scales with flat rims, 0.19–0.26 µm in diameter, formed the inner layer; whereas, scales with upright rims, 0.25–0.45 µm in diameter, formed the outer layer. Both scales had a faint cross in the centre of the scale face, and the radiating ridges extended from the faint cross to the periphery. Molecular phylogenetic analysis indicated that the SSU and LSU rDNA sequences from our symbiont fell within the clade ofChrysochromulina. However, our symbiont differed in the details of the patterning on the scale face from the other species ofChrysochromulina. Thus, we proposeC. andersonii sp. nov. for the symbiotic alga from the polycystine radiolarianD. truncatum.
孤立放射虫中黄棕色共生体(Primnesiida)的精细结构及其与类似棘鱼共生体的比较1
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