Lactose Enhances Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus
Lactose Enhances Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus
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DOI：
10.1263/jbb.106.115
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发表时间：
2008-08-01
影响因子：
2.8
通讯作者：
Sawayama, Shigeki
中科院分区：
文献类型：
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作者：
Fang, Xu;Yano, Shinichi;Sawayama, Shigeki
解纤维素顶孢菌是一种产生纤维素酶的真菌，已被酶工业开发利用。为了促进A. cellulolyticus菌株C-1生产纤维素酶，我们评估了糖类：Solka Floe（纤维素）、可溶性大豆多糖（SSPS）、支链淀粉、乳糖、海藻糖、槐糖、纤维二糖、半乳糖、山梨糖、乳糖酸和混合物作为纤维素酶生产碳源的效果。含有 SSPS 的 Solka Floe 增强了纤维素酶的产量。乳糖作为唯一碳源诱导该真菌中纤维素酶的合成，并观察到乳糖和 Solka Floe 之间的协同效应。分析了添加或不添加乳糖的培养物产生的各种酶活性和粗酶的蛋白质组成。结果表明，添加乳糖大大提高了A. cellidolyticus生产各种具有纤维素酶活性的蛋白质。据我们所知，这是关于解纤维放线菌中乳糖生产纤维素酶的第一份报告。
Acremonium cellulolyticus is a fungus that produces cellulase and has been exploited by enzyme industry. To promote cellulase production by A. cellulolyticus strain C-1, we evaluated the effects of the saccharides: Solka Floe (cellulose), soluble soybean polysaccharide (SSPS), pullulan, lactose, trehalose, sophorose, cellobiose, galactose, sorbose, lactobionic acid, and mixtures as carbon sources for cellulase production. Solka Floe with SSPS enhanced cellulase production. Lactose as the sole carbon source induced cellulase synthesis in this fungus, and the synergistic effects between lactose and Solka Floe was observed. Various enzyme activities and the protein composition of crude enzyme produced by cultures with or without addition of lactose were analyzed. The results showed that lactose addition greatly improves the production of various proteins with cellulase activity by A. cellidolyticus. To our knowledge, this is the first report on production of cellulases by lactose in the A. cellulolyticus.