SCHISTOSOMA-MANSONI - MIGRATION AND ATTRITION OF IRRADIATED AND CHALLENGE SCHISTOSOMULA IN THE MOUSE
SCHISTOSOMA-MANSONI - MIGRATION AND ATTRITION OF IRRADIATED AND CHALLENGE SCHISTOSOMULA IN THE MOUSE
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DOI：
10.1017/s0031182000052446
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发表时间：
1983-01-01
期刊：
影响因子：
2.4
通讯作者：
WILSON, RA
中科院分区：
文献类型：
--
作者：
MASTIN, AJ;BICKLE, QD;WILSON, RA
使用定量组织学技术在小鼠中研究了经辐照的免疫尾蚴和攻击血吸虫的脂肪，该技术可能比以前使用的组织切碎和孵育恢复技术更有效地估计皮肤和肺部的寄生虫数量。有证据表明，经辐射（20克拉德）的寄生虫在皮肤中的迁移略有延迟，但皮肤中血吸虫的死亡似乎可以忽略不计。大多数受辐射的寄生虫至少在感染后第 21 天仍保留在肺部；此时观察到的大多数血吸虫均已死亡。在用受辐射尾蚴免疫的小鼠中，没有证据表明皮肤中的攻击感染有磨损。免疫动物肺部中攻击寄生虫的迁移特征与初始动物肺部中正常寄生虫的迁移特征没有显着差异。在肺部观察到死亡的攻击寄生虫，但认为它们的数量不足以解释通过灌注测量的最终损耗。攻击血吸虫的剩余消耗可能发生在全身和/或肝门循环中。
The fat of irradiated, immunizing cercariae and challenge schistosomula was investigated in mice using a quantitative, histological technique, which may be more efficient in estimating parasite numbers in skin and lungs than the tissue mincing and incubation recovery technique used previously. There was evidence for slight retardation of irradiated (20 krad) parasite migration in skin, but death of schistosomula in the skin appeared negligible. The majority of irradiated parasites remained in the lungs until at least day 21 after infection; most schistosomula observed at this time were dead. In mice immunized with irradiated cercariae there was no evidence of attrition of the challenge infection in the skin. The migration profile of challenge parasites in the lungs of immunized animals was not significantly different from that of normal parasites in the lungs of naive animals. Dead challenge parasites were observed in the lungs but their numbers were not considered great enough to account for the final attrition measured by perfusion. The remainder of the attrition of challenge schistosomula may occur in the systemic and/or hepatic portal circulation.