Rapid Mounting of Zebrafish Larvae for Brain Imaging.
Rapid Mounting of Zebrafish Larvae for Brain Imaging.
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快速安装斑马鱼幼虫进行脑成像。
DOI：
10.1089/zeb.2021.0062
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发表时间：
2021
期刊：
影响因子：
2
通讯作者：
Peterson,RandallT
中科院分区：
文献类型：
--
作者：
Geng,Yijie;Peterson,RandallT
脑成像需要将斑马鱼幼虫垂直安装，但麻醉或固定的幼虫在没有外部支撑的情况下往往会侧倒。目前的解决方案是手动固定单个幼虫直至液体琼脂糖凝固，这既费时又费力，而且对初学者不友好。我们开发了一种使用计算机数控制造的模具在琼脂糖凝胶中形成幼虫形槽的方法。每个插槽几乎完美地适合直立位置的幼虫，并且可以通过插入插槽轻松安装幼虫。平均而言，使用这种方法可以在 <1 分钟内安装每个幼虫。
Brain imaging requires mounting of zebrafish larvae in a vertical position, but anesthetized or fixed larvae tend to fall on their sides without external support. Current solution is to manually hold individual larva until liquid agarose solidifies, which is time consuming, labor intensive, and unfriendly to beginners. We developed a method to form larva-shaped slots in agarose gel using a computer numerical controlled manufactured mold. Each slot nearly perfectly fits a larva in its upright position, and larvae can be easily mounted by inserting into the slots. On average, each larva can be mounted in <1 min using this method.