Geovibrio ferrireducens, a phylogenetically distinct dissimilatory Fe(III)-reducing bacterium
Geovibrio ferrireducens, a phylogenetically distinct dissimilatory Fe(III)-reducing bacterium
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DOI：
10.1007/s002030050340
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发表时间：
1996-06-01
影响因子：
2.8
通讯作者：
McInerney, MJ
中科院分区：
文献类型：
--
作者：
Caccavo, F;Coates, JD;McInerney, MJ
一种新的，遗传学上不同的，异化，铁（III）-还原细菌分离自碳氢化合物污染的沟渠表面沉积物。该分离株命名为PAL-1，是一种专性厌氧、非发酵、能动的革兰氏阴性弧菌。PAL-1生长在含有乙酸盐作为电子供体和焦磷酸铁、羟基氧化铁、柠檬酸铁、Co（III）-EDTA或元素硫作为唯一电子受体的限定培养基中。PAL-1还使用脯氨酸、氢、乳酸盐、丙酸盐、琥珀酸盐、富马酸盐、丙酮酸盐或酵母提取物作为Fe（III）还原的电子供体。它是已知的第一个将氨基酸的氧化与Fe（III）还原偶联的细菌。PAL-1不还原氧、Mn（IV）、U（VI）、Cr（VI）、硝酸盐、硫酸盐、亚硫酸盐或硫代硫酸盐，乙酸盐作为电子供体。PAL-1的细胞悬浮液表现出连二亚硫酸盐还原减去空气氧化的差异光谱，是C型细胞色素的特征。PAL-1的16 S rRNA基因序列的分析表明，该菌株是不相关的变形菌门中的任何描述的金属还原细菌，并与Flexistipes sinusarabici，形成一个单独的线内的细菌下降。在表型和遗传学上，菌株派-1不同于所有其他描述的细菌，并且代表新属和种的模式菌株，铁还原地弧菌。
A new, phylogenetically distinct, dissimilatory, Fe(III)-reducing bacterium was isolated from surface sediment of a hydrocarbon-contaminated ditch. The isolate, designated strain PAL-1, was an obligately anaerobic, nonfermentative, motile, gram-negative vibrio. PAL-1 grew in a defined medium with acetate as electron donor and ferric pyrophosphate, ferric oxyhydroxide, ferric citrate, Co(III)-EDTA, or elemental sulfur as sole electron acceptor. PAL-1 also used proline, hydrogen, lactate, propionate, succinate, fumarate, pyruvate, or yeast extract as electron donors for Fe(III) reduction. It is the first bacterium known to couple the oxidation of an amino acid to Fe(III) reduction. PAL-1 did not reduce oxygen, Mn(IV), U(VI), Cr(VI), nitrate, sulfate; sulfite, or thiosulfate with acetate as the electron donor. Cell suspensions of PAL-1 exhibited dithionite-reduced minus air-oxidized difference spectra that were characteristic of c-type cytochromes. Analysis of the 16S rRNA gene sequence of PAL-1 showed that the strain is not related to any of the described metal-reducing bacteria in the Proteobacteria and, together with Flexistipes sinusarabici, forms a separate line of descent within the Bacteria. Phenotypically and phylogenetically, strain PAI-1 differs from all other described bacteria, and represents the type strain of a new genus and species, Geovibrio ferrireducens.