Differentiation of flavonoid glycoside isomers by using metal complexation and electrospray ionization mass spectrometry
Differentiation of flavonoid glycoside isomers by using metal complexation and electrospray ionization mass spectrometry
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DOI：
10.1016/j.jasms.2003.07.002
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发表时间：
2003-12-01
影响因子：
3.2
通讯作者：
Brodbelt, JS
中科院分区：
文献类型：
--
作者：
Pikulski, M;Brodbelt, JS
类黄酮异构体的解析是通过电喷雾电离串联质谱（ESI-MS/MS）通过形成和碰撞活化解离（CAD）的金属/类黄酮络合物含有辅助配体。添加金属盐和合适的中性辅助配体的黄酮类化合物在溶液中的结果在形成[M（II）（类黄酮-H）配体]（+）复合物的ESI，碰撞活化解离后，往往会导致更独特的碎片模式比观察到的常规质子化或去质子化的黄酮类化合物。以前，2，2 '-联吡啶被用作辅助配体，现在我们比较和探索使用替代的吡啶基配体，包括4，7-二苯基-1，10-菲咯啉。使用这种技术，三组黄酮苷异构体的区别，包括芹菜素，槲皮素，和毛地黄黄酮苷。(C)2003年美国质谱学会。
The elucidation of flavonoid isomers is accomplished by electrospray ionization tandem mass spectrometry (ESI-MS/MS) via formation and collisional activated dissociation (CAD) of metal/flavonoid complexes containing an auxiliary ligand. Addition of a metal salt and a suitable neutral auxiliary ligand to flavonoids in solution results in the formation of [M(II) (flavonoid-H) ligand](+) complexes by ESI which, upon collisional activated dissociation, often result in more distinctive fragmentation patterns than observed for conventional protonated or deprotonated flavonoids. Previously, 2,2'-bipyridine was used as an auxiliary ligand, and now we compare and explore the use of alternative pyridyl ligands, including 4,7-diphenyl-1,10-phenanthroline. Using this technique, three groups of flavonoid glycoside isomers are differentiated, including glycosides of apigenin, quercetin, and luteolin. (C) 2003 American Society for Mass Spectrometry.